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ELISA Assistant

TGFβ2 Emax® ImmunoAssay System

Technical Literature
Product Improvements
Samples Tested
Cross-Reactivity
Catalog

The TGFβ2 Emax® ImmunoAssay System provides optimized reagents and a protocol for the sensitive and specific detection of biologically active TGFβ2. After an overnight coating of a 96 well plate, the specific protein is detected using an antibody sandwich format. The system uses horseradish peroxidase-conjugated secondary antibody for the final chromogenic detection of bound TGFβ2.

  • Specificity: Specific detection of respective factor; less than 5% cross-reactivity with other related growth factors (TGFβ1 and TGFβ3).
  • Sensitivity: Detect at least 32pg/ml
  • Standard Range: 15.6–1000pg/ml
Recommended Plate: Nunc Maxisorp™, Dynex Immulon®-4 and Costar plates

Technical Literature

The complete protocol is found in Technical Bulletin #TB224.


Product Improvements

The Emax® ImmunoAssay Systems have been improved to provide longer shelf-life and ease of use. The key improvements include newly formulated horseradish peroxidase (HRP) conjugates that provide increased stability and easier dilutions, and a new ready-to-use, single-component TMB substrate. The entire system is now stored at –20°C for up to 6 months from the date of receipt, before thawing and using. Once thawed the product should be stored at 4°C for up to three months.

System

Size Old Cat.#
(Prior to Mar 2001)
New Cat.#
(Since Mar 2001)

TGFβ2 Emax® ImmunoAssay System

5 Plate G3400 G7600

Also see: Emax® ImmunoAssay Systems: New and Improved for Ease of Use and Reliability (Neural Notes 19)


Samples Tested

The TGFβ2 Emax® ImmunoAssay System has been used on a wide variety of tissues. For information about sample types which have been used with the TGFβ2 Emax® ImmunoAssay System see the list of Citations for TGFβ2 Emax® ImmunoAssay System.

Cross-Reactivity

For information regarding species cross-reactivity, please select one of the following species for a list of citations: