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Technical Literature
Product Improvements
Samples Tested
Cross-Reactivity
Catalog
The TGFβ1 Emax® ImmunoAssay System provides optimized reagents
and a protocol for the sensitive and specific detection of transforming growth factor
β1 (TGFβ1). After an overnight coating of a 96 well plate, the specific protein is
detected using an antibody sandwich format. The system uses horseradish peroxidase-conjugated
secondary antibody for the final chromogenic detection of bound TGFβ1.
- Specificity:Specific detection of respective factor; less than 3%
cross-reactivity with other related growth factors (TGFβ2 and TGFβ3).
- Sensitivity: Detect at least 32pg/ml
- Standard Range: 15.6–1000pg/ml
Recommended Plate: Nunc Maxisorp™, Dynex Immulon®-4 and Costar plates
Technical Literature
The complete protocol is found in Technical Bulletin #TB196.
Product Improvements
The Emax® ImmunoAssay Systems have been improved to provide
longer shelf-life and ease of use. The key improvements include newly formulated
horseradish peroxidase (HRP) conjugates that provide increased stability and easier
dilutions, and a new ready-to-use, single-component TMB substrate. The entire system is
now stored at –20°C for up to 6 months from the date of receipt, before thawing and
using. Once thawed the product should be stored at 4°C for up to three months.
System |
Size |
Old
Cat.#
(Prior to Mar 2001) |
New
Cat.#
(Since Mar 2001) |
TGFß1 Emax®
ImmunoAssay System |
2 x 96 wells |
did not exist |
G7590 |
| 5 x 96 wells |
G1230 |
G7591 |
Also see: Emax® ImmunoAssay Systems: New and Improved for
Ease of Use and Reliability (Neural Notes 19)
Samples Tested
The TGFβ1 Emax® ImmunoAssay System has been used on a wide
variety of tissues. For information about sample types which have been used with
the TGFβ1 Emax® ImmunoAssay System see the list of
Citations
for TGFβ1 Emax® ImmunoAssay System.
Cross-Reactivity
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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