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Technical Literature
Product Improvements
Samples Tested
Cross-Reactivity
Catalog
The NT-3 Emax® ImmunoAssay Systems provide optimized reagents
and a protocol for the sensitive and specific detection of neurotrophin-3 (NT-3). After an
overnight coating of a 96-well plate, the specific protein is detected using an antibody
sandwich format. The systems use horseradish peroxidase-conjugated secondary antibody for
the final chromogenic detection of bound neurotrophic factor.
- Specificity: Specific detection of respective factor; less than 3%
cross-reactivity with other related neurotrophic and growth factors.
- Sensitivity: Detection down to at least 9.4pg/ml.
- Standard Range: 4.7-300pg/ml
Recommended Plate: Nunc Maxisorp™ or Dynex Immulon®-4 plates
Technical Literature
The complete protocol is found in Technical Bulletin
#TB243.
Product Improvements
The Emax® ImmunoAssay Systems have been improved to provide
longer shelf-life and ease of use. The key improvements include newly formulated
horseradish peroxidase (HRP) conjugates that provide increased stability and easier
dilutions, and a new ready-to-use, single-component TMB substrate. The entire system is
now stored at –20°C for up to 6 months from the date of receipt, before thawing and
using. Once thawed the product should be stored at 4°C for up to three months.
System |
Size |
Old
Cat.#
(Prior to Mar 2001) |
New
Cat.#
(Since Mar 2001) |
NT-3 Emax®
ImmunoAssay System |
2 x 96 wells |
G3510 |
G7640 |
| 5 x 96 wells |
G3370 |
G7641 |
Also see: Emax® ImmunoAssay Systems: New and Improved for
Ease of Use and Reliability (Neural Notes 19)
Samples Tested
The NT-3 Emax ImmunoAssay System has been used on a wide
variety of tissues. For information about sample types which have been used with
the NT-3 Emax® ImmunoAssay System see the list of Citations
for NT-3 Emax® ImmunoAssay System.
Cross-Reactivity
For information regarding species cross-reactivity, please select one of the following species for a list of citations:
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