|
Technical Literature
Product Improvements
Samples Tested
Cross-Reactivity
Catalog
The NGF Emax® ImmunoAssay Systems provide optimized reagents and
a protocol for the sensitive and specific detection of biologically active nerve growth
factor (NGF). After an overnight coating of a 96 well plate, the specific protein is
detected using an antibody sandwich format. The systems use horseradish
peroxidase-conjugated secondary antibody for the final chromogenic detection of bound
neurotrophic factor. The system can be used to quantitate NGF in tissue culture
supernatants and tissue extracts in the range of 7.8–500pg/ml. Avoid using samples
containing high levels of IgG such as serum, plasma and spleen.
- Specificity: Specific detection of respective factor; less than 3%
cross-reactivity with other related neurotrophic and growth factors.
- Sensitivity: Detects a minimum of 15.6pg/ml of NGF.
- Standard Range: 7.8–500pg/ml
- Recommended Plate: Nunc Maxisorp™, Dynex Immulon®-4 and
Costar plates
Technical Literature
The complete protocol is found in Technical Bulletin
#TB226
- Emax® ImmunoAssay Systems in Print (Neural Notes 14)
- Acid Treatment of NGF Samples. (Neural Notes 7)
- Detection of Increased Tissue Concentrations of Nerve Growth Factor with an Improved
Extraction Procedure (Neural Notes 7)
- Product Update: Emax® ImmunoAssay System Family (Neural Notes 7)
Product Improvements
The Emax® ImmunoAssay Systems have been improved to provide
longer shelf-life and ease of use. The key improvements include newly formulated
horseradish peroxidase (HRP) conjugates that provide increased stability and easier
dilutions, and a new ready-to-use, single-component TMB substrate. The entire system is
now stored at –20°C for up to 6 months from the date of receipt, before thawing and
using. Once thawed the product should be stored at 4°C for up to three months.
System |
Size |
Old
Cat.#
(Prior to Mar 2001) |
New
Cat.#
(Since Mar 2001) |
NGF Emax® ImmunoAssay
System |
2 x 96 wells |
G3550 |
G7630 |
| 5 x 96 wells |
G3560 |
G7631 |
Also see: Emax® ImmunoAssay Systems: New and Improved for
Ease of Use and Reliability (Neural Notes 19)
Samples Tested
The NGF Emax® ImmunoAssay System has been used on a wide
variety of tissues. For information about sample types which have been used with
the NGF Emax® ImmunoAssay System see the list of Citations
for NGF Emax® ImmunoAssay System.
Cross-Reactivity
For information regarding species cross-reactivity, please select one of the following species for a list of citations:
|
