The GDNF Emax® ImmunoAssay Systems provide optimized reagents
and protocol for the sensitive and specific detection of glial cell-line-derived
neurotrophic factor (GDNF). After an overnight coating of a 96 well plate, the specific
protein is detected using an antibody sandwich format. The systems use horseradish
peroxidase-conjugated secondary antibody for the final chromogenic detection of bound
neurotrophic factor.
Specificity: Specific detection of respective factor; less than 3%
cross-reactivity with other related neurotrophic and growth factors.
Sensitivity: Detects a minimum of 32 pg/ml
Standard Range: 15.6–1000pg/ml
Recommended Plate: Nunc MaxiSorp™ and Dynex Immulon®-4
Technical Literature
The complete protocol is found in Technical Bulletin
#TB221.
The Emax® ImmunoAssay Systems have been improved to provide longer shelf-life and ease
of use. The key improvements include newly formulated horseradish peroxidase (HRP)
conjugates that provide increased stability and easier dilutions, and a new ready-to-use,
single-component TMB substrate. The entire system is now stored at –20°C for up to 6
months from the date of receipt, before thawing and using. Once thawed the product should
be stored at 4°C for up to three months except for the GDNF Standard which should always
be stored at –20°C.
System
Size
Old
Cat.#
(Prior to Mar 2001)
New
Cat.#
(Since Mar 2001)
GDNF Emax®
ImmunoAssay System
2 x 96 wells
G3520
G7620
5 x 96 wells
G3240
G7621
Also see: Emax® ImmunoAssay Systems: New and Improved for
Ease of Use and Reliability (Neural Notes 19)
Samples Tested
The GDNF Emax® ImmunoAssay System has been used on a wide
variety of tissues. For information about sample types which have been used with
the GDNF Emax® ImmunoAssay System see the list of Citations
for GNDF Emax® ImmunoAssay System.
Cross-Reactivity
For information regarding species cross-reactivity please select one of the
following species for a list of citations:
