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Technical Literature
Product Improvements
Samples Tested
Cross-Reactivity
Catalog
The BDNF Emax® ImmunoAssay Systems provide optimized reagents
and protocol for the sensitive and specific detection of brain-derived neurotrophic factor
(BDNF). After an overnight coating of a 96 well plate, the specific protein is detected
using an antibody sandwich format. The systems use a horseradish peroxidase-conjugated
secondary antibody for the final chromogenic detection of bound neurotrophic factor.
- Specificity: Specific detection of BDNF; typically less than 3% cross-reactivity
with other related neurotrophic factors (NGF, NT-3 and NT-4) at 100ng/ml
- Sensitivity: Detects a minimum of 15.6pg/ml of BDNF.
- Standard Range: 7.8–500pg/ml
Recommended Plate: Nunc MaxiSorp™, Dynex Immulon®-4, Dynex Immulon®-2,
& Costar Plates
Technical Literature
The complete protocol is found in Technical Bulletin
#TB257.
Product Improvements
The Emax® ImmunoAssay Systems have been improved to provide
longer shelf-life and ease of use. The key improvements include newly formulated
horseradish peroxidase (HRP) conjugates that provide increased stability and easier
dilutions, and a new ready-to-use, single-component TMB substrate. The entire system is
now stored at –20°C for up to 6 months from the date of receipt, before thawing and
using. Once thawed the product should be stored at 4°C for up to three months.
System |
Size |
Old
Cat.#
(Prior to Mar 2001) |
New
Cat.#
(Since Mar 2001) |
BDNF Emax®
ImmunoAssay System |
2 x 96 wells |
G6991 |
G7610 |
| 5 x 96 wells |
G6981 |
G7611 |
Also see: Emax® ImmunoAssay Systems: New and Improved for
Ease of Use and Reliability (Neural Notes 19)
Samples Tested
The BDNF Emax ImmunoAssay System has been used on a wide
variety of tissues. For information about sample types which have been used with
the BDNF Emax® ImmunoAssay System see the list of Citations
for BNDF Emax® ImmunoAssay System.
Cross-Reactivity
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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