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Molar Ratio of Insert to Vector

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Generally ligations are tested at 1:3, 1:1, and 3:1 insert:vector molar ratios.

Length of insert (in kb)
Amount of vector (in ng)
Length of vector (in kb)
  You need ng of insert for a 1:1 molar ratio
length of insert (in kb)   x  ng of vector  =  ng of insert needed for a 1:1 ratio

length of vector (in kb)