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Linear DNA: Micrograms to Picomoles of DNA Ends

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Amount of DNA (in g)
Size of DNA (in kb)
  You have pmol DNA ends
g DNA  x  pmol  x  106pg  x  1  x  2  x  kb  =  pmol DNA ends




660pg 1g N 1000bp

N is the number of nucleotides (in kb),
660pg/pmol is the average molecular weight of a single nucleotide pair,
2 is the number of ends in a linear DNA molecule, and
kb/1000bp is a conversion factor for kilobases to base pairs