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Anti-βIII Tubulin mAb (Cat.# G7121) is a protein G-purified IgG1 monoclonal antibody
(from clone 5G8) raised in mice against a peptide (EAQGPK) corresponding to the C-terminus
of βIII tubulin. It is directed against βIII tubulin, a specific marker for
neurons. The major use of this antibody is for labeling neurons in tissue sections and
cell culture. The antibody has been tested to perform in frozen and paraffin-embedded
sections of rat brain, cerebellum and spinal cord, human and rat fetal CNS progenitor cell
cultures and adult human paraffin-embedded brain.
- Antibody Form: Mouse monoclonal IgG1 (clone 5G8), 1mg/ml in
PBS containing no preservatives.
- Immunogen: Peptide corresponding to the C-terminus (EAQGPK) of
βIII tubulin.
- Specificity: Cross-reacts with most mammalian species. Does not label
non-neuronal cells (e.g., astrocytes).
Promega Product Information Sheet (.pdf, 31kb): 
Western Blotting
Citations
for Western Blot Analysis
Immunocytochemistry
Technical Literature:
- Total RNA isolation from neuronal cultures using Promega's SV 96 Binding Plate on the
Biomek® 2000. Neural Notes 19,
15.—Fluorescent microscopy of the human neuroblastoma cell line SH-SY5Y. Cells were fixed with 3.7% paraformaldehyde, probed with the Anti-ßIII Tubulin mAb at
1.0μg/ml, and developed with an Alexa Fluor™-594-conjugated goat anti-mouse IgG from Molecular Probes.
- Gillessen,T., Tokai, F. and Breiter, K. (2000) Specific labeling of neurons and glia in
mixed cerebrocortical cultures. Neural Notes 18,
10–11.—Protocols for immunostaining with the Anti-βIII Tubulin mAb
and Anti-GFAP pAb in rat cultures.
- Imaging with Promega Reagents No. 7. Neural
Notes 15, 8–9
(.pdf, 419kb).—Undifferentiated PC12 cells
were immunolabeled for βIII tubulin. Cells were stained with 1μg/ml Anti-βIII Tubulin mAb,
and visualized with Anti-Mouse Cy3™-conjugate.
- Imaging with Promega Reagents No. 6. Neural
Notes 14, 10–11
(.pdf, 189kb).–Cerebral cortex culture
were immunolabeled
for βIII tubulin. Dissociated cerebral cortex cultures were
double labeled for GFAP and βIII tubulin. The Anti-βIII
Tubulin mAb was used at 1:200 dilution and recognized with an anti-mouse Texas
Red-conjugate from Jackson ImmunoResearch.
- Imaging with Promega Reagents No. 4. Neural
Notes 12, 12–13
(.pdf, 142kb).—Rat neural progenitor cells are shown
migrating away from a cluster of apoptotic cells. Cells were labeled with the Anti-βIII
Tubulin mAb, Apoptosis Detection System, Fluorescein and DAPI. The reactivity with
the antibody was visualized with an anti-mouse Cy3™-conjugate from Jackson
ImmunoResearch.
Citations
for Immunocytochemistry
Immunohistochemistry
Technical Literature:
- Imaging with Promega Reagents No. 10. Neural Notes
18, 8–9
(.pdf, 1,439kb).
—Double-immunostaining shows
the presence of ubiquitinated ataxin-1 nuclear
inclusions and immunostained βIII tubulin—IHC of mouse brain
sections referenced to Vig, P.J.S. et al. (2000) J. Neurol. Sci. 174,
100–110.
-
Imaging with Promega Reagents No. 8. Neural
Notes 16
(.pdf, 296kb), 10–11.
—Immunohistochemical detection of βIII
tubulin was performed in mouse cortical granule neurons using the Anti-βIII Tubulin mAb.
-
Imaging with Promega Reagents No. 3. Neural
Notes 11, 12–13
(.pdf, 142kb).
—Immunofluorescence staining of rat
cerebellum for βIII tubulin. Staining is demonstrated on frozen rat
brain sections and formalin-fixed, paraffin-embedded rat brain sections.
Citations
for Immunohistochemistry
Other Applications
No other applications have been published.
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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