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Neurotrophin-3, a 27kDa homodimer that shares high sequence homology with NGF, BDNF and
NT-4/5, influences many neuron types in the central and peripheral nervous system. NT-3 is
also highly conserved across species. Anti-Human NT-3 pAb (Cat.# G1651) is generated in chickens and
purified using the EGGstract® IgY Purification System. IgY, the 180kDa chicken
IgG homolog, can be produced in chickens against certain biological antigens that fail to
elicit a humoral immune response in rabbits or other mammals due to species relatedness.
Therefore, the antibody is highly specific for NT-3 in a variety of mammalian species.
- Immunogen: Human recombinant NT-3.
- Antibody Form: Chicken IgY is provided at 0.5mg/ml in 0.1M NaCl, 0.01M
K2HPO4 and 50μg/ml gentamicin.
- Specificity: Cross-reactive with human and mouse NT-3 and is presumed
to cross-react with rat and Rhesus monkey NT-3 based on factor sequence identity across
species; does not cross-react with BDNF or NGF and has limited cross-reactivity to NT-4.
Promega Product Information Sheet (.pdf, 32kb): 
Western Blotting
Technical Literature:
- Promega's recently release neurotrophic factors and antibodies. Neural Notes 1, 6–13.
Citations
for Western Blot Analysis
ELISA
NT-3 Emax® ImmunoAssay System
—The Anti-Human NT-3 pAb is one of the two anti-NT-3 antibodies used in the immunoassay.
Technical Literature:
Immunocytochemistry
Technical Literature:
- Vickland, H. and Silver, J. (1995) Immunostaining glial cells with Promega's Anti-Human
Neurotrophin Antibodies. Neural Notes 2,
8–9.
- Immunostaining with Promega Antibodies. Neural Notes
9, 12–13 (.pdf, 298kb). Protocol appeared on inside front cover of Neural Notes 5.
Complete Protocol
- Imaging With Promega Reagents. No.7. Neural Notes 15, 8–9
(.pdf, 419kb). —Immunolabeling of NT-3 in teased
nerves and cultured Schwann cells.
Referenced to Meier, C., et al. (1999) J. Neurosci. 19, 3847–59
Citations
for Immunocytochemistry
Immunohistochemistry
No protocols currently exist from Promega
Citations
for Immunohistochemistry
Other Applications
Citations
for Other Applications
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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