Antibody Assistant - Immunostaining of Monocyte-derived Macrophages and Human Fetal Microglial Cells With the Anti NT-3 pAb

Catalog | Cart | Log In

Antibody Assistant

Immunostaining of Monocyte-derived Macrophages and Human Fetal Microglial Cells With the Anti NT-3 pAb

Culture microglia cells on coverslips.
Remove media and gently wash cells 2 times with PBS.
Fix cells in 4% paraformaldehyde at room temperature for 30 minutes.
Wash cells 2 times with PBS.
Apply Promega's Anti-Human NT-3 pAb, diluted 1:100 in Common Antibody Diluent, (CAD; Biogenex Laboratories).
Incubate at room temperature on an orbital shaker for 2 hours.
Wash cells 3 times with PBS.
Apply rabbit anti-chicken IgY–DTAF conjugate (Jackson ImmunoResearch Laboratories) diluted 1:100 in CAD.
Protect from light and incubate at room temperature for 30 minutes.
Rinse cells 1 time with PBS and mount on glass slides with Gelvatol fluorescent mounting medium.