|
Anti-HaloTag® pAb (Cat.# G9281) is a rabbit polyclonal antibody
designed for use in
immunocytochemistry and Western blot applications. Anti-HaloTag® pAb can detect
HaloTag® protein expression in situ.
- Immunogen: HaloTag® protein.
- Antibody Form: Rabbit polyclonal IgG at 1mg/ml in PBS.
- Specificity: The Anti-HaloTag® pAb is specific for HaloTag® protein and exhibits low cross-reactivity with
E. coli and mammalian cell extracts.
Technical Manual : HaloTag® Technology: Focus on Imaging
Western Blotting
Part of the Quality control assay for this antibody is recognition of 5ng of HaloTag® protein on a Western
blot with a 1:1,000 dilution.
Immunocytochemistry
Technical Literature:
- Learish, R. (2007) A polyclonal HaloTag® antibody for Western blotting and immunocytochemistry
Promega
Notes 95, 23–24.—ICC of HEK293 cells stably expressing p65 and HaloTag® protein.
Immunohistochemistry
No protocols have been developed at Promega for IHC
Other Applications
No other applications have been developed for this antibody at Promega
Cross-Reactivity
The antibody was raised to HaloTag® protein and has low cross-reactivity with E. coli and mammalian cell extracts.
|
