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Anti-ACTIVE® p38 pAb, Rabbit (Cat.# V1211), is an
affinity-purified polyclonal antibody that recognizes the active form of p38 enzyme. The
Anti-ACTIVE® p38 pAb is raised against the dually phosphorylated peptide
sequence representing the catalytic core of the active p38 enzyme. The phosphorylated
amino acid residues correspond to Thr 180 and Tyr 182 of the p38 enzyme.
- Specificity: Preferentially detects the dually-phosphorylated, active
form of the p38 kinase.
- Immunogen: Dually phosphorylated Thr/Gly/Tyr region (pTGpY) derived
from the catalytic core of the active form of p38 kinase, corresponding to Thr182 and
Tyr184 of the mammalian p38 enzyme.
- Antibody Form: Affinity-purified Rabbit IgG; supplied in PBS (pH 7.4).
- Value: When used at the recommended 1:2,000 dilution, this product will
generate up to 200ml of blotting solution, sufficient for 20 Western blots of 10ml each.
Promega Product Information Sheet (.pdf, 33kb): 
Western Blotting
Protocol:
- Technical Bulletin #TB262: Anti-ACTIVE®
MAPK, p38 and JNK Polyclonal Antibodies and Anti-ACTIVE® Qualified Secondary
Antibody Conjugates.—Contains Promega's optimized protocol for Western analysis of
extracts and has different optimized protocols for PVDF membranes or nitrocellulose
membranes.
Technical Literature:
- Jarvis, B.W. and O'Brien, M. (1999) PC12 Extracts for use with new Anti-pT183
MAPK and Anti-ACTIVE® Antibodies. Promega
Notes 72, 10–12.—Western Blots of new Sorbitol-treated
and Untreated PC12 Cell Extracts (Cat. #V8100).
- Using Anti-ACTIVE® MAPK, p38 and JNK pAbs for Western blotting. Neural Notes 18, 12–13.
Citations
for Western Blot Analysis
Immunocytochemistry
Protocol:
- Technical Bulletin #TB262: Anti-ACTIVE®
MAPK, p38 and JNK Polyclonal Antibodies and Anti-ACTIVE® Qualified Secondary
Antibody Conjugates.—Contains Promega's optimized protocol for immunocytochemical
analysis of p38 in PC12 cells treated with sorbitol.
Technical Literature:
- Immunostaining with the new Anti-ACTIVE® p38 Antibody. Neural Notes 17, 2-4.—ICC
of sorbitol-treated PC12 cells.
Citations
for Immunocytochemistry
Immunohistochemistry
Technical Literature:
- Hoffman, R., O'Brien, M. and Jarvis, B.W. (2001) Immunohistochemical staining using
Promega Anti-ACTIVE® and Apoptosis Antibodies. Cell Notes 1, 4–7.—IHC
of rat brain hippocampus with the Anti-ACTIVE® JNK pAb and Anti-ACTIVE®
p38 pAb. Full protocols are provided.
Citations
for Immunohistochemistry
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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