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Anti-ACTIVE® JNK pAb, Rabbit (Cat.# V7931) is an
affinity-purified polyclonal antibody that recognizes the dually phosphorylated, active
form of JNK (c-Jun N-terminal protein kinase), also known as SAPK (Stress-Activated
Protein Kinase). Anti-ACTIVE® JNK pAb is raised against a dually
phosphorylated peptide sequence representing the catalytic core of the active JNK enzyme.
The phosphorylated amino acid residues correspond to Thr 183 and Tyr 185 of the JNK2
enzyme.
- Specificity: Preferentially detects the dually phosphorylated, active
form of the stress-activated protein kinase (SAPK), also known as c-Jun N-terminal kinase,
JNK.
- Immunogen: Dually phosphorylated Thr/Pro/Tyr region (pTPpY) derived
from the catalytic core of the active form of JNK kinase, corresponding to Thr183 and
Tyr185 of the mammalian JNK2 enzyme.
- Antibody Form: Affinity purified Rabbit IgG; supplied in 10mM sodium
phosphate, 20mM NaCl (pH 7.4).
- Value: Anti-ACTIVE® JNK pAb is available in two convenient
sizes. Cat.# V7931 will generate up to 200ml of blotting solution, sufficient for 20
Western blots of 10ml each. The larger size, Cat.# V7932 will generate up to 600ml of
blotting solution, sufficient for 60 Western blots of 10ml each.
Promega Product Information Sheet (.pdf, 37kb): 
Western Blotting
Protocol:
- Technical Bulletin #TB262: Anti-ACTIVE®
MAPK, p38 and JNK Polyclonal Antibodies and Anti-ACTIVE® Qualified Secondary
Antibody Conjugates–Contains Promega's optimized protocol for Western analysis of
extracts and has different optimized protocols for PVDF membranes or nitrocellulose
membranes.
Technical Literature:
- Jarvis, B.W. and O'Brien, M. (1999) PC12 Extracts for use with new Anti-pT183
MAPK and Anti-ACTIVE® Antibodies. Promega
Notes 72, 10–12.—Western blots of new Sorbitol-treated
and untreated PC12 Cell Extracts (Cat.# V8100).
- Using Anti-ACTIVE® MAPK, p38 and JNK pAbs for Western blotting.
Neural Notes 18, 12–13.
Citations
for Western Blot Analysis
Immunocytochemistry
Protocol:
- Technical Bulletin #TB262:Anti-ACTIVE®
MAPK, p38 and JNK Polyclonal Antibodies and Anti-ACTIVE® Qualified Secondary
Antibody Conjugates.–Contains Promega's optimized protocol for immunocytochemical
analysis of JNK in PC12 cells treated with Sorbitol.
Technical Literature:
- Immunostaining with the new Anti-ACTIVE® p38 Antibody. Neural Notes 17, 2–4.–ICC
of Sorbitol-treated PC12 cells.
- Imaging with Promega Reagents No. 9. Neural Notes 17,
10 (.pdf, 303kb).—Tau-1 staining patterns are altered in
apoptotic cells (PC12) possessing active JNK in the nucleus.–Referenced to
Davis, P.K. and Johnson, G.V.W.(1999) J. Biol. Chem.
274, 35686–92.
Citations
for Immunocytochemistry
Immunohistochemistry
Technical Literature:
Hoffman, R., O'Brien, M. and Jarvis, B.W. (2001) Immunohistochemical staining using
Promega Anti-ACTIVE® and Apoptosis Antibodies. Cell Notes 1, 4–7.—IHC
of rat brain hippocampus with the Anti-ACTIVE® JNK pAb and Anti-ACTIVE®
p38 pAb. Full protocols are provided.
Citations
for Immunohistochemistry
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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