This is the protocol used by Promega R&D on human bladder tissue.

1. Obtain paraffin blocks of tissue samples and cut 6.0μm sections.
2. Deparaffinize the section as follows:
   
   1. Wash 2 times for 5 minutes each in either xylene or Hemo De (xylene substitute)
   2. Soak 3 minutes in 100% ethanol
   3. Soak 3 minutes in 95% ethanol
   4. Soak 3 minutes in 70% ethanol
   5. Wash 2 times for 2 minutes each in PBS
      
3. Block the sections for 30 minutes in PBS containing 1.0% BSA.
4. Incubate with a 1:100 dilution of the Anti-Human Tryptase mAb-Biotin Conjugate in PBS containing 1.0% BSA for 60 minutes at room temperature. Dilution may require optimization with different tissues and different samples.
5. Wash 3 times for 3 minutes each time with PBS.
6. Incubate sections for 60 minutes with a streptavidin-Cy3 conjugate (Jackson Immunoresearch, Cat. #016-160-084) diluted 1:2000 in PBS.
7. Wash 3 times for 3 minutes each time with PBS.
8. Mount in Vectashield^® Mounting Media with DAPI (Vector Laboratories, Cat. #H1200).