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Anti-pS473 Akt pAb (Cat.#G7441) is an affinity-purified polyclonal rabbit
antibody. The antibody is purified using a phosphorylated peptide that corresponds to the
phospho-Ser473 form of Akt-1.
- Specificity: The antibody is selective for the Ser473 phosphorylated isoforms of
Akt and does not show cross-reactivity with nonphosphorylated Akt.
- Immunogen: Peptide from the singly phosphorylated Ser473 region from the
C-terminus of Akt-1 protein.
- Antibody Form: Affinity purified rabbit IgG, supplied in PBS with 50μg/ml
gentamicin.
- Value: Will generate 100ml of blotting solution, sufficient for 10 Western blots
of 10ml each.
Promega Product Information Sheet (.pdf, 173kb): 
Western Blotting
Protocol:
The recommended conditions are provided on the Promega Product
Information Sheet (.pdf, 173kb) which accompanies the antibody.
Technical Literature:
Somberg, R., O'Brien, M. and Haak-Frendscho, M. (2000) A strategy for survival: The Akt
signaling cascade. Promega Notes 74,
21–22.—Westerns of mouse NIH3T3 cell extracts.
Immunocytochemistry
Technical Literature:
- Somberg, R., O'Brien, M. and Haak-Frendscho, M. (2000) A strategy for survival: The Akt
signaling cascade. Promega Notes 74,
21–22.—ICC of human Jurkat cells.
Citations
for Immunocytochemistry
Immunohistochemistry
Technical Literature:
- Hoffman, R., O'Brien, M. and Jarvis, B.W. (2001) Immunohistochemical staining using
Promega Anti-ACTIVE® and Apoptosis Antibodies. Cell Notes 1, 4–7.—IHC
of frozen rat brain hippocampal sections with the Anti-pS473 Akt pAb.
Full protocols are provided.
Other Applications
No other applications have been developed for this antibody at Promega
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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