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p75 neurotrophin receptor (p75NTR), also known as low affinity NGF receptor,
LNGFR, and p75LNGFR, binds nerve growth factor, brain-derived neurotrophic
factor, neurotrophin-3 and neurotrophin-4 with varying specificities. p75NTR
plays an important role in neurotrophic factor signaling including neuronal apoptosis.
Anti-Human p75 pAb (Cat.# G3231) provides a valuable tool for
understanding the role of p75NTR in neuronal death. The antibody is produced for Promega by the lab of Moses Chao.
- Immunogen: Cytoplasmic domain of the human p75 neurotrophin receptor.
- Antibody Form: Purified rabbit IgG; 1mg/ml in PBS containing 50μg/ml
gentamicin.
- Specificity: Human, rat, mouse and chicken p75 as determined by Moses
Chao.
Promega Product Information Sheet (.pdf, 32kb): 
Immunocytochemistry
Technical Literature:
- Immunostaining with Promega Antibodies. Neural Notes
9,
12–14 (.pdf, 298kb). —Immunofluorescence staining for p75NTR in human fetal CNS preparation.
Promega Protocol
- Imaging with Promega Reagents No.7. Neural Notes
15, 8–9 (.pdf,
419kb).—PC12 cells immunolabeled for p75NTR.
- Imaging with Promega Reagents No. 9. Neural Notes
17, 10–11
(.pdf, 303kb).—Double-labeled
immunofluorescence of neurites extending from an explant plated on Matrigel™.
Referenced to Tisay, K.T. and Key, B. (1999) J. Neurosci. 19, 9890–9.
Immunohistochemistry
Technical Literature:
- Imaging with Promega Reagents No. 2. Neural Notes
10, 12–13
(.pdf, 516kb).—Electron micrograph (15,000X) demonstrating
immunostaining in the inner molecular layer of the rat dentate gyrus.
- Imaging with Promega Reagents No. 5. Neural Notes
13, 10–11
(.pdf, 148kb).—Cells bearing the p75LNTR were
identified in 5μm frozen sections of erbB2-targeted mice with Promega's Anti-Human
p75 pAb and apoptotic cells were identified by TUNEL staining. Reprinted from
Britsch, S., et al. (1998) Genes Dev. 12, 1825–35.
Citations
for Immunohistochemistry
Other Applications
Technical Literature:
- Anti-Human p75 Polyclonal Antibody. Neural Notes
3, 10.—Immunoprecipitation from PC12 cells and rat brain extracts.
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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