|
The purified Anti-VAChT (Vesicular Acetylcholine Transporter) pAb (Cat.# G4481) is raised in goats
against a peptide (CSPPGPFDGCEDDYNYYSRS) corresponding to amino acids 511–530 of the
carboxy terminus of the cloned rat VAChT. It is a novel tool to identify functional
cholinergic neurons in the central and peripheral nervous system where the antibody stains
fibers and neuronal cell bodies. This antibody has application for the study of the
pathophysiology of neurodegenerative diseases involving the cholinergic system and for
mapping cholinergic neurons in the nervous system.
- Immunogen: Carboxy-terminal peptide sequence 511–530 corresponding to
cloned rat VAChT protein.
- Antibody Form: Purified goat polyclonal IgG; 0.5mg/ml in PBS containing
no preservatives.
- Specificity: Cross-reacts with VAChT in rat and mouse, but not in
human, guinea pig, rabbit or cat.
Promega Product Information Sheet (.pdf, 31kb): 
Western Blotting
Not tested for this application
Immunohistochemistry
Protocol:
Promega Protocol
Technical Literature:
- Anti-VAChT pAb. Neural Notes 10,
11.—Immunofluorescent staining for VAChT protein in ventral gray matter of rat
spinal cord.
- Imaging with Promega Reagents No. 2. Neural
Notes 10, 12–13
(.pdf, 516kb).
—Immunofluorescent staining of
vesicular acetylchonine transporter (VAChT) in rat tongue showing motor end plates and rat
spinal cord ventral horn.
Other Applications
No other applications identified as yet
|
