|
Tryptase, a 135kDa tetrameric serine protease, is the principal protein mediator
component of mast cell granules and is abundant in both degranulated and unstimulated mast
cells. It therefore is an exquisite marker in heterogeneous populations of mast cells. The
Anti-Human Tryptase mAb Biotin (Cat.# G3361) allows for positive phenotypic identification of mast cells
in disease of many organs and has been qualified in Western blot analysis, ELISA and
immunocytochemistry applications with complex biological fluids. This antibody is both
more specific and sensitive than standard metachromic staining procedures for mast cells.
Furthermore, it is effective for a wide range of both Carnoy's and formaldehyde-fixed
tissues. The biotinylated tryptase mAb allows for amplification of signal with a
streptavidin conjugate (e.g., HRP or FITC) while maintaining very low background from
nonspecific reactivity, a problem often associated with use of secondary antibodies.
- Immunogen: Purified human lung tryptase.
- Antibody Form: IgG1 (clone AA5), affinity purified; supplied as 1mg/ml
in PBS containing no preservatives.
- Specificity: No cross-reactivity to related biomolecules; cross-reacts in
non-human primates.
Promega Product Information Sheet (.pdf, 32kb): 
Western Blotting
Technical Literature:
- Niles, A.L., et al. (1999) Recombinant human mast cell tryptase. Promega Notes 70, 11–13.—Westerns
of recombinant versus natural human tryptase.
Citations
for Western Blot Analysis
ELISA
The antibody has been used in an indirect ELISA to recognize rhTryptase.
The antibody was used with another antibody in a sandwich ELISA to quantitate tryptase
by Okragly, A.J. et al. (1999) Elevated tryptase, nerve growth factor,
neurotrophin-3 and glial cell line-derived neurotrophic factor levels in the urine of
interstitial cystitis and bladder cancer patients. J. Urol. 161,
438–441. The other antibody described in the paper, an IgY, is not commercially available from Promega
or any company.
Immunohistochemistry
Protocol:
Promega's Protocol
Technical Literature:
- Anti-Tryptase mAb-Biotin Conjugate: Marker for neuroimmunological studies. Neural Notes 10, 14.—IHC of
normal human bladder tissue.
- Niles, A.L., et al. Recombinant human mast cell tryptase. Promega Notes 70, 11–13.—IHC
of cancerous human bladder tissue.
Other Applications
No other applications have been developed for this antibody at Promega
Cross-Reactivity Citations
|
