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TrkB is the high-affinity receptor for brain-derived neurotrophic factor (BDNF) and
neurotrophin-4 (NT-4). Two forms of the TrkB receptor exist, the full-length, active
145kDa protein and a truncated, nonsignaling 95kDa protein. The truncated isoform lacks
the cytoplasmic tyrosine kinase catalytic region and therefore does not signal in response
to BDNF or NT-4. The Anti-TrkB In pAb (Cat.# G1561) is a polyclonal antibody raised in chickens against
a cytoplasmic domain of the carboxy terminus of the mouse TrkB intracellular domain.
- Immunogen: A fusion protein containing residues 482–501
(HISNGSNTPSSSEGGPDAVI) derived from a portion of the intracellular domain of mouse TrkB
generated in E. coli using a proprietary synthetic carrier protein technology.
- Antibody Form: Chicken IgY provided at 0.5mg/ml in 0.1M NaCl, 0.01M K2PO4
(pH 7.4).
- Specificity: Detects full-length TrkB, the high-affinity receptor for
BDNF and NT-4, in human, rat and primate brain.
Promega Product Information Sheet (.pdf, 30kb): 
Western Blotting
Not recommended because of lack of reactivity. Experiments were attempted on rat whole brain lysates. The blots were
clean. We believe it is a case of lack of antigen rather than failure of the antibody. No further optimization was
performed.
Immunocytochemistry
Citations
for Immunocytochemistry
Immunohistochemistry
Protocol:
Promega Protocol
Technical Literature:
- Anti-TrkB In pAb: Antibody specific for the intracellular domain of TrkB receptor. Neural Notes 10, 14.—IHC
of rat cerebellum Purkinje cells.
Citations
for Immunohistochemistry
Other Applications
No other applications have been identified at this time.
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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