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Nerve growth factor (NGF) is a member of the neurotrophin family of growth factors. NGF
is expressed in sympathetic and sensory-innervated peripheral tissues and mediates
phosphorylation of specific intracellular proteins. At the cellular level, NGF expression
has been demonstrated in lymphocytes, smooth muscle cells, epithelial cells, astrocytes,
fibroblasts and Schwann cells. Anti-NGF mAb (Cat.# G1131) was designed as a specific marker of NGF in
Western blotting, ELISA and immunostaining applications.
- Immunogen: Purified murine NGF, 2.5S.
- Antibody Form: Rat IgG (clone 1G3) provided at 1mg/ml as frozen liquid
in PBS containing no preservatives.
- Specificity: Reacts with human NGF, 2.5S mNGF and to a lesser extent
with 7S mNGF. Cross-reacts between mammalian species.
- Activity: Anti-NGF exhibits a half-maximal titer of less than or equal
to 250ng/ml in an ELISA protocol using 100ng of 2.5S mNGF (Cat.# G5141).
Promega Product Information Sheet (.pdf, 31kb):
Western Blotting
Citations
for Western Blot Analysis
ELISA
NGF Emax® Immunoassay System —The Anti-NGF mAb is one of the two anti-NGF antibodies in the system.
Technical Literature:
Immunocytochemistry
No protocols available.
Immunohistochemistry
Antibodies to Neurotrophic Factors. Neural Notes 1,
9. —Immunostaining of urinary bladder from adult, female guinea pig sensitized and repeatedly
challenged with bovine serum albumin.
Citations
for Immunohistochemistry
Other Applications
The Anti-NGF mAb is not a neutralizing antibody
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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