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Anti-GFAP pAb (Cat.# G5601) is a polyclonal antibody against
glial fibrillary acidic protein (GFAP),a specific marker of astrocytes in the central nervous system, and is qualified
for immunostaining applications.
- Immunogen: Purified glial fibrillary acidic protein from bovine spinal
cord.
- Antibody Assistant - Anti-GFAP pAb Resources
- Antibody Form: Purified rabbit IgG; supplied at 1mg/ml in PBS
containing 50μg/ml gentamicin.
- Specificity: Human, bovine and rat GFAP; not recommended for mouse.
Promega Product Information Sheet (.pdf, 31kb): 
Western Blotting
Part of the quality control is a Western blot detection of 10ng of recombinant bovine
GFAP.
Citations for
Western Blotting
Immunocytochemistry
Technical Literature:
- Imaging with Promega Reagents No. 8. Neural
Notes 16, 10–11 (.pdf, 296kb).—Staining of astrocytes by Anti-GFAP
pAb in rat neural progenitor cell cultures.
Complete Protocol
- Gillessen,T., Tokai, F. and Breiter, K. (2000) Specific labeling of neurons and glia in
mixed cerebrocortical cultures. Neural Notes 18,
10–11.—Protocols for immunostaining with the Anti-βIII Tubulin mAb and
Anti-GFAP pAb in rat culture.
Citations for Immunocytochemistry
Immunohistochemistry
Technical Literature:
- Hoffman, R. O'Brien, M. and Jarvis, B.W. (2001) Immunohistochemical staining using
Promega Anti-ACTIVE® and apoptosis antibodies. Cell
Notes 1, 4–7.—IHC of frozen rat brain hippocampal
sections probed with the Anti-GFAP pAb to show that Anti-ACTIVE® MAPK pAb
labels a subset of astrocytes. Protocols are detailed.
Other Applications
No other applications have been identified.
Cross-Reactivity Citations
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