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Human glial cell-line-derived neurotrophic factor (GDNF), a 30kDa homodimer, has been
shown to be a potent survival factor for a variety of neurons. The receptor complex for
GDNF has been elucidated, though members of the multicomponent receptor family continue to
grow. With applications in Western blotting, ELISA and immunostaining, Promega's
Anti-Human GDNF pAb (Cat.# G2791) is a useful tool to continue
the investigation of GDNF's role in multiple facets of neurological systems.
- Immunogen: Human recombinant GDNF.
- Antibody Form: Chicken IgY; 0.5mg/ml in 0.1M NaCl, 0.01M K2HPO4,
50μg/ml gentamicin.
- Specificity: Cross-reactive between mammalian species; does not
cross-react with TGFα, TGFβ1, NGF or BDNF at up to 10μg/ml.
Promega Product Information Sheet (.pdf, 32kb): 
Western Blotting
Technical Literature:
- Promega's recently release neurotrophic factors and antibodies. Neural Notes 1, 6–13.
- Emax® ImmunoAssay Systems: Frequently Asked Questions
Neural Notes 10, 8–9. Figure
1, p.9 is from Okragly, A.J. and Haak-Frendscho, M. (1997) An acid treatment method for the enhanced
detection of GDNF in biological samples. Exp. Neurol. 145,
1–5.
Citations
for Western Blot Analysis
ELISA
Technical Literature:
Immunocytochemistry
No protocols or citations currently exist.
Immunohistochemistry
Technical Literature:
- Imaging with Promega Reagents No. 6. Neural Notes
14, 10–11 (.pdf, 189kb). —Immunohistochemistry was
perfomed on 5μm paraffin sections. Promega's Anti-Human GDNF pAb (Cat.# G2791) was
used at 1:500 dilution; a rabbit anti-chicken IgY (1:160) conjugated to rhodamine was used
as the secondary antibody.
Citations
for Immunohistochemistry
Other Applications
Citations for Other Applications
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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