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Ciliary Neurotrophic Factor (CNTF) is a 23kDa protein shown to exert effects on a wide
number of peripheral and central nervous system cell types. CNTF is structurally unrelated
to the neurotrophins (nerve growth factor, brain-derived neurotrophic factor,
neurotrophin-3 and neurotrophin-4) and uses a signaling pathway distinct from the Trk
pathways utilized by these factors. Anti-Rat CNTF pAb (Cat.# G1631) provides a useful tool for
understanding CNTF's effects by Western blotting, immunostaining and ELISA applications.
- Immunogen: Rat recombinant CNTF.
- Antibody Form: Chicken IgY, provided at 0.5mg/ml as frozen liquid in
0.1M NaCl, 0.01M K2HPO4 and 50μg/ml gentamicin.
- Specificity: Cross-reactive between mammalian species; does not
cross-react with other neurotrophic factors.
Promega Product Information Sheet (.pdf, 32kb): 
Western Blotting
Technical Literature:
- Promega's recently release neurotrophic factors and antibodies. Neural Notes 1, 6–13.
Citations
for Western Blot Analysis
ELISA
Technical Literature:
Immunocytochemistry
Technical Literature:
- Immunostaining with Promega Antibodies. Neural Notes
9, 12–14 (.pdf, 298kb).
—Central nervous system cells were immunostained with the Anti-CNTF pAb at a concentration of
5–10μg/ml, diluted in PBS + 3% goat serum.
Complete protocol
Immunohistochemistry
Citations
for Immunohistochemistry
Other Applications
No other applications reported
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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