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BDNF, a 27kDa homodimer originally derived from human brain and which shares high
sequence homology with NGF, NT-3 and NT-4/5, influences many neuron types in the
central nervous system (1,2). Anti-Human BDNF pAb (Cat.# G1641) is generated in chickens and purified using the EGGstract®
IgY Purification System (Cat.# G2610).
IgY, the 180kDa chicken IgG homolog, can be produced in chickens against certain
biological antigens that fail to elicit a humoral immune response in rabbits or other
mammals due to species relatedness. Therefore, the antibody is highly specific for BDNF.
- Immunogen: Human recombinant BDNF.
- Antibody Form: Chicken IgY, provided at 0.5mg/ml in 0.1M NaCl, 0.01M K2HPO4
and 50μg/ml gentamicin.
- Specificity: Cross-reactive between mammalian species; does not
cross-react with other neurotrophic factors.
Promega Product Information Sheet (.pdf, 32kb): 
Western Blotting
Technical Literature:
Promega's recently release neurotrophic factors and antibodies. Neural Notes 1, 6–13.
Citations
for Western Blot Analysis
ELISA
BDNF
Emax® ImmunoAssay System: The Anti-Human BDNF pAb is one of the
two anti-BDNF antibodies used in the ELISA System.
Technical Literature:
Citations for
ELISA
Immunocytochemistry
Protocol:
- Vickland, H. and Silver, J. (1995) Immunostaining Glial Cells with Promega's Anti-Human
Neurotrophin Antibodies. Neural Notes 2,
8–9.
—Immunostaining of human fetal microglia.
Complete Protocol
Technical Literature:
- Immunostaining with Promega Antibodies. Neural Notes 9,
12–14 (.pdf, 298kb). Details on hippocampal neuron isolation, culture and
staining can be found in Goodman, L. et al. (1996) Mol. Cell. Neurosci.
7, 222–38.
- Immunostaining with Promega Antibodies No.3. Neural
Notes 11, 12–13
(.pdf, 142kb).
Details on cell cultures and immunostaining can be found in Tongiorgi, E.,
Righi, E. and Cattaneo, A. (1997)
J. Neurosci. 17, 9492–505.
- Imaging with Promega Reagents No. 8. Neural
Notes 16, 10–11
(.pdf, 296kb). Details on cell cultures and
immunostaining may be found in Leventhal, C. et al. (1999) Mol. Cell.
Neurosci. 13, 450–64.
Citations
for Immunocytochemistry
Immunohistochemistry
Technical Literature:
- Immunostaining with Promega Antibodies No. 2. Neural
Notes 10, 12–13 (.pdf, 516kb). —Information communicated to Promega by
a researcher for staining
protocol. Tissue was perfused with 2.0% paraformaldehyde with minimal post fixation (i.e. start
the IHC as soon as possible). Incubate tissue section with 0.1% Triton® X-100 in PBS for 30 minutes (optimization is
necessary). Incubate with 10–15μg/ml Anti-Human BDNF pAb.
Other Applications
- Toma, J.G. and Kaplan, D.R. (1998) Neutralization of TrkB tyrosine kinase receptor
activity using BDNF pAb. Neural Notes 9,
20.
Citations
for Other Applications
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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