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The Anti β-Galactosidase mAb (Cat.# Z3781) [subclass IgG2a
κ was purified from ascites of
a mouse hybridoma and recognizes E. coli β-galactosidase.
- Immunogen: β-galactosidase.
- Antibody Form: 2.0–2.5mg/ml in 10mM Tris-HCl (pH 8.0), 150mM NaCl, 0.02% sodium
azide.
- Specificity: E. coli β-galactosidase near the C-terminal end.
Promega Product Information Sheet (.pdf, 32kb): 
Western Blotting
Technical Literature:
- Larson, G. (1992) Western blotting using the ProtoBlot® II AP System with
Stabilized Substrate. Promega Notes 35,
18–20.—Westerns of purified β-galactosidase and TNT® Coupled
Reticulocyte Lysate-expressed β-galactosidase.
Citations
for Western Blot Analysis
Immunocytochemistry
Citations
for Immunocytochemistry
Immunohistochemistry
Technical Literature:
- Imaging with Promega Reagents No. 4. Neural
Notes 12, 12–13
(.pdf, 142kb).—IHC of
transgenic Drosophila embryos. Referenced to Zhou, L. et al. (1997) Proc. Natl. Acad. Sci. USA 94,
5131–5136.
- Imaging with Promega Reagents. No. 6. Neural
Notes 14, 10–1
(.pdf, 189kb).—IHC of rat
retina referenced to Takahashi, M. et al. (1998) Mol. Cell. Neurosci. 12, 340–348.
- Imaging with Promega Reagents, No. 7. (1999) Neural
Notes 15, 8–9 (.pdf,
419kb).—IHC of transgenic mouse olfactory bulb referenced to
Rodriquez, J., Feinstein, P. and Mombaerts, P. (1999) Cell 97, 199–208.
Citations
for Immunohistochemistry
Immunoprecipitation
Citations
for Other Applications
Cross-Reactivity
Raised to E. coli β-galactosidase. Cross-reactivity to
mammalian species does not apply.
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