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Poly (ADP-ribose) polymerase (PARP), a nuclear enzyme involved in DNA
repair, is a well-known substrate for caspase-3 cleavage during apoptosis. Anti-PARP p85
Fragment pAb(a) (Cat.# G7341) is a rabbit polyclonal antibody specific for the p85 fragment
of PARP that results from caspase cleavage of the 116kDa intact molecule and thus provides
an in situ marker for apoptosis. Each batch of antibody is quality assurance tested for
use in immunostaining applications and contains sufficient antibody for 50
immunocytochemical reactions at the suggested working dilution of 1:100.
- Immunogen: N-terminal peptide from p85 fragment.
- Antibody Form: Affinity-purified rabbit polyclonal antibody provided in
Dulbecco's PBS.
- Specificity: Specifically detects PARP p85 fragment in human and bovine cells and
rat tissues. Does not recognize the 116kDa intact PARP protein.
Promega Product Information Sheet (.pdf, 32kb): 
Western Blotting
Protocol:
- Technical Bulletin #TB273, Anti-PARP p85 Fragment
pAb. The protocol is in the legend of Figure 2.
Technical Literature:
- Anti-PARP p85 Fragment pAb: Novel apoptosis marker specific for p85 fragment of PARP.
Neural Notes 14, 12.—Westerns
of recombinant, cleaved bovine PARP and apoptotic human Jurkat cell extracts.
Citations
for Western Blot Analysis
Immunocytochemistry
Protocol:
- Technical Bulletin #TB273, Anti-PARP p85 Fragment pAb.
Technical Literature:
- Anti-PARP p85 Fragment pAb: Novel apoptosis marker specific for p85 fragment of
PARP. Neural Notes 14, 12.—ICC
of human Jurkat cells.
Citations
for Immunocytochemistry
Immunohistochemistry
Technical Literature:
- Hoffman, R., O'Brien, M. and Jarvis, B.W. (2001) Immunohistochemical staining using
Promega Anti-ACTIVE® and Apoptosis Antibodies. Cell Notes 1, 4–7.—IHC
of paraffin-embedded postnatal day 0 mouse brain sections.
- Knaapen, M., et al. (2000) Cleaved PARP as a marker for
apoptosis in tissue sections. Promega Notes
72, 7–9.—IHC of human atherosclerotic plaques.
Citations for Immunohistochemistry
Other Applications
During the development of the antibody, a beta-tester reported successful use of
the Anti-PARP p85 Fragment pAb in flow cytometry. For more information regarding the use
of this antibody in flow cytometry, please contact Promega
Technical Services.
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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