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Anti-Luciferase pAb (Cat.# G7451) is a goat polyclonal antibody designed for use in
immunocytochemistry and Western blot applications. Anti-Luciferase pAb can detect
luciferase enzyme expression in situ.
- Immunogen: Recombinant luciferase from North American firefly (Photinus
pyralis).
- Antibody Form: Goat polyclonal IgG at 1mg/ml in PBS containing 50μg/ml
gentamicin.
- Specificity: Anti-Luciferase pAb is specific for firefly luciferase (Photinus
pyralis) and does not cross-react with sea pansy (Renilla reniformis)
luciferase.
Promega Product Information Sheet (.pdf, 31kb): 
Western Blotting
Part of the Quality control assay for this antibody is recognition of 2ng of recombinant firefly luciferase on a Western
blot with a 1:1,000 dilution.
Citations
for Western Blot Analysis
Immunocytochemistry
Protocol:
- Part of the Quality control assay for this antibody is ICC of human HeLa cells stably
transfected with a luciferase construct.
Promega's Protocol
Technical Literature:
- Imaging with Promega Reagents, No. 7. (1999) Neural
Notes 15, 8–9 (.pdf,
419kb).—ICC of NIH3T3 cells transiently expressing luciferase.
Citations
for Immunocytochemistry
Immunohistochemistry
No protocols have been developed at Promega for IHC
Citations
for Immunohistochemistry
Other Applications
No other applications have been developed for this antibody at Promega
Cross-Reactivity
The antibody was raised to firefly luciferase. Does not cross react with
Renilla luciferase. Mammalian species cross-reactivity is not applicable.
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