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Anti-ERK 1/2 pAb (Cat.# V1141) is a polyclonal antibody purified from rabbit serum. The
antibody is affinity-purified using a peptide sequence in human/rat ERK1.
- Specificity: Detects ERK1 and ERK2 in the nonphosphorylated, monophosphorylated
and dually phosphorylated forms.
- Immunogen: Sequence representing a conserved region in human and rat ERK1 located
outside of the catalytic core and the activation domain of the enzyme.
- Antibody Form: Affinity-purified rabbit IgG; supplied in PBS (pH 7.4).
- Value: When used at the recommended 1:5,000 dilution, this product will generate
up to 200ml of blotting solution, sufficient for 20 Western blots of 10ml each.
Promega Product Information Sheet (.pdf, 25kb): 
Western Blotting
Protocol:
- Recommended blotting method is provided on the second page of the Promega Product
Information Sheet accompanying the antibody. Essentially the blotting method is the same as that for
the Anti-ACTIVE® MAPK pAb in Technical Bulletin #TB262.
Technical Literature:
- Jarvis, B.W. and Moravec, R. (1998) New Anti-ACTIVE® MAPK & 'pan ERK 1/2'
Antibodies for Western Analysis. Promega
Notes 69, 9–10.—Western blots of NGF-treated PC12 cell
extracts.
Citations
for Western Blot Analysis
Immunocytochemistry
Protocol:
Promega's Protocol
Citations
for Immunocytochemistry
Immunohistochemistry
Technical Literature:
- Hoffman, R., O'Brien, M. and Jarvis, B.W. (2001) Immunohistochemical staining using
Promega Anti-ACTIVE® and Apoptosis Antibodies. Cell Notes 1, 4–7.—IHC
of rat brain hippocampus with the Anti-ACTIVE® MAPK pAb and Anti-ERK 1/2 pAb.
Full protocols are provided.
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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