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Anti-Cytochrome c mAb (Cat.# G7421) is a monoclonal IgG antibody (clone 6H2.B4) against
cytochrome c [Goshorn, S.C., Retzel, E. and Jemmerson, R. (1991) J. Biol. Chem. 266,
2134–42.], an electron carrier protein identified as essential to the mitochondrial
respiratory process. This protein was recently shown to be an important molecule in the
apoptosis pathway. Cytochrome c is translocated from the mitochondria to the cytoplasm
where it associates with other apoptosis-inducing proteins to activate caspase-9. The main
application of the antibody is for immunofluorescence studies.
- Immunogen: Rat cytochrome c.
- Antibody Form: Mouse monoclonal IgG (clone 6H2.B4), 1mg/ml in PBS
containing 50μg/ml gentamicin.
- Specificity: Specific to the native form of rat, mouse, and human cytochrome c.
Promega Product Information Sheet (.pdf, 29kb): 
Immunocytochemistry
Protocol:
- Immunocytochemistry is a quality-control assay for the antibody.
Promega's Protocol
Technical Literature:
- Anti-Cytochrome c mAb (2000) Neural Notes 15,
11.—ICC of Jurkat cells.
- Apoptosis FAQs: What differentiates an apoptotic cell from a normal cell with regard to
immunostaining with Anti-Cytochrome c mAb? (2000) Neural
Notes 16, 18 (.pdf,
195kb).—ICC of anti-Fas mAb treated and
untreated human Jurkat cells.
Citations
for Immunocytochemistry
Immunohistochemistry
Protocol:
Cross-Reactivity Citations
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