Anti-ACTIVE® Caspase-3 pAb (Cat.# G7481) is intended for use as a marker of
apoptosis; it specifically stains apoptotic cells without staining non-apoptotic cells.
Includes sufficient antibody to perform 25 immunocytochemical stainings (100μl
of a 1:250 dilution of the antibody per staining).
Immunogen: Peptide derived from the p17 fragment of caspase-3 having sequence
homology in human, mouse, rat and hamster.
Antibody Form: Affinity-purified rabbit IgG; supplied in Dulbecco's PBS.
Specificity: Specifically recognizes the cleaved active form of caspase-3 in
human and mouse.
Promega Product Information Sheet (.pdf, 111kb):
Immunocytochemistry
Protocol:
Recommended ICC method is provided on the second page of the Promega Product
Information Sheet (.pdf, 111kb) accompanying the antibody.
Technical Literature:
Riss, T., Moravec, R. and O'Brien, M. (2000) Anti-ACTIVE® Caspase-3 pAb for
detection of apoptosis. Promega Notes75,
17–19.—ICC of mouse L929 fibroblast cells and human SH-SY5Y neuroblastoma
cells.
Hoffman, R., O'Brien, M. and Jarvis, B.W. (2001) Immunohistochemical staining using
Promega Anti-ACTIVE® and Apoptosis Antibodies. Cell Notes 1, 4–7.—IHC
of paraffin-embedded postnatal day 0 mouse brain sections. Full protocols provided.
