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Anti-ACTIVE® CaM KII pAb (Cat.# V1111) is a polyclonal antibody (pAb)
directed against the multifunctional
calcium/calmodulin-dependent protein kinase CaM kinase II (CaM KII) that is phosphorylated
on threonine 286 (pT286). The Anti-ACTIVE® CaM KII pAb was raised
against the phosphothreonine-containing peptide.
- Specificity: Preferentially detects CaM KII autophosphorylated on Threonine
286.
- Immunogen: Threonine-phosphorylated peptide corresponding to the phosphorylated
Thr286 region of the mammalian calcium/calmodulin-dependent protein kinase.
- Antibody Form: Affinity purified rabbit IgG; supplied in 10mM sodium
phosphate, 20mM NaCl (pH 7.4).
- Value: When used at the recommended dilution of 1:5,000, it generates up to 200ml
of blotting solution sufficient for 20 Western blots of 10ml each.
Promega Product Information Sheet (.pdf, 33kb) 
Western Blotting
Protocol:
- Anti-ACTIVE®
CaM KII pAb, (pT286) and Anti-ACTIVE® Qualified Secondary Antibody
Conjugates, Technical Bulletin #TB264.
Notes: Optimized protocol for Western blotting with the antibody.
Technical Literature:
- Goueli, S. (1998) Anti-ACTIVE® Antibody for Specific
Detection of Phosphorylated CaM KII Protein Kinase. Promega Notes 67, 9–11.
- Goueli, S. (2000) Tools to study the activation of CaM KII in neuronal functions.
Promega Notes 76, 6–9.
- Atkins, C.M. and Sweat, J.D. (1999) Activation of α-CaM KII During Mammalian
Associative Learning. Neural Notes 13,
17–19.
Citations
for Western Blot Analysis
Immunocytochemistry
Technical Literature:
-
Goueli, S., O'Brien, M. and Hsaio, K. (1998) Anti-ACTIVE™ Antibody for Specific
Detection of Phosphorylated CaM KII Protein Kinase. Promega Notes 67, 9–11.
- Imaging with Promega Reagents No. 8. (2000) Neural
Notes 16, 10–11
(.pdf, 296kb).
- Imaging with Promega Reagents No. 5. (1999) Neural
Notes 13, 10–11
(.pdf, 148kb).
Citations
for Immunocytochemistry
Immunohistochemistry
Technical Literature:
- Hoffman, R., O'Brien, M. and Jarvis, B.W. (2001) Immunohistochemical staining
using Promega Anti-ACTIVE® and Apoptosis Antibodies. Cell Notes 1, 4–7.
Cross-Reactivity Citations
For information regarding species cross-reactivity, please select one of the
following species for a list of citations:
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