Thermostable DNA Polymerases

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Technical Reference

Properties of Thermostable DNA Polymerases


Organism	Taq* (Thermus aquaticus)	Tfl (Thermus flavus)	Tth (Thermus thermophilus)	Tli (Thermococcus litoralis)	Pfu** (Pyrococcus furiosus)
MW	80kDa	94kDa	94kDa	90kDa	92kDa
Extension Temperature	74°C	74°C	74°C	74°C	75°C
5'→3' Exonuclease Activity	Yes	Yes	Yes	No	No
3'→5' Exonuclease Activity	No	No	No	Yes	Yes
Reverse Transcriptase Activity	Weak	Yes	Yes	No	N/A
Predominant PCR Product Ends	3'-A	3"-A	3'-A	70% Blunt; 30% Single-base overhangs	Blunt

GoTaq^® DNA Polymerase and PCR Master Mix contain Taq* DNA polymerase.
**Not available in North America.
N/A = Not available.

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