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RNA POLYMERASE PROMOTER PRIMERS |
|
Primer Sequence |
Cat.# |
Tm (°C) |
Applicable Vectors |
|
SP6
5'-d(TATTTAGGTGACACTATAG)-3' |
Q5011 |
42 |
pGEM®-1, -2, -3, -4, -3Z, -4Z, -3Zf(+/–), -5Zf(+/–), -7Zf(+/–), -9Zf(–),
-11Zf(+/–),
-13Zf(+); pGEM®-luc; pSP-luc+; pSP-luc+ NF; pGEMEX®-1, 2; pSP64,
64Poly(A),
-70, -71, -72, -73; pALTER®-1, -Ex1, -Ex2; LambdaGEM®-11, -12; pGEM®-T,
T-Easy; LambdaGEM®-2, -4; PinPoint™ Xa-1, -2, -3; PinPoint™ Xa-1
T-Vector |
T7
5´-d(TAATACGACTCACTATAGGG)-3´ |
Q5021 |
47 |
pGEM®-1, -2, -3, -4, -3Z, -4Z, -3Zf(+/–), -5Zf(+/–), -7Zf(+/–), -9Zf(–),
-11Zf(+/–),
-13Zf(+); pGEM®-luc; pSP-luc+; pSP-luc+ NF; pGEMEX®-1, 2; pSP64,
64Poly(A),
-70, -71, -72, -73; pALTER®-1, -Ex1, -Ex2; LambdaGEM®-11, -12; pGEM®-T,
T-Easy; LambdaGEM®-2, -4; PinPoint™ Xa-1, -2, -3; PinPoint™ Xa-1
T-Vector |
T3
5´-d(ATTAACCCTCACTAAAGGGA)-3´ |
Q5741 |
50 |
pACT, pALTER®-MAX; pBIND; pGEMEX®-1,
-2;
pCI-neo; HaloTag® pHT2 |
T7 EEV
5´-d(AAGGCTAGAGTACTTAATACGA)-3´ |
Q6700 |
50 |
pALTER®-MAX; pCI; pSI; pCI-neo; pCMVTNT™;pTNT; phMGFP; HaloTag®
pHT2; psiCHECK™-1, -2; |
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pUC/M13 PRIMERS |
|
Primer Sequence |
Cat.# |
Tm (°C) |
Applicable Vectors |
Forward (17mer) (-40)
5´-d(GTTTTCCCAGTCACGAC)-3´ |
Q5391 |
50 |
pGEM®-3Z, -4Z, -3Zf(+/–), -5Zf(+/–), -7Zf(+/–), -9Zf(–),
-11Zf(+/–),
-13Zf(+); pGEM®-luc; pGEM®-T,
T-Easy; pALTER®-1,
-Ex1, -Ex2; pCAT®-Basic, -Promoter, -Enhancer, -Control; pGeneClip™ Basic, Puromycin, Hygromycin, Neomycin, hMGFP; psiSTRIKE™ Basic, Puromycin, Hygromycin,
Neomycin; psiLentGene™ Basic, Puromycin, Hygromycin, Neomycin; |
Forward (24mer) (-47)
5´-d(CGCCAGGGTTTTCCCAGTCACGAC)-3´
|
Q5601 |
64 |
same as Forward (17mer) |
Reverse (17mer)
5´-d(CAGGAAACAGCTATGAC)-3´ |
Q5401 |
47 |
pGEM®-3Z, -4Z, -3Zf(+/–), -5Zf(+/–), -7Zf(+/–), -9Zf(–), -11Zf(+/–),
-13Zf(+); pGEM®-luc ; pSP64, pSP64 Poly(A), pSP65; pGEM®-T,
-T Easy; pCAT®-Basic,-Promoter, -Enhancer, -Control;
pALTER-1, Ex-1, Ex-2; pGeneClip™-Basic, Puromycin, Hygromycin,
Neomycin; hMGFP; psiSTRIKE™ Basic, Puromycin, Hygromycin, Neomycin; psiLentGene™ Basic Puromycin, Hygromycin, Neomycin |
Reverse (22mer)
5´-d(TCACACAGGAAACAGCTATGAC)-3´
|
Q5421 |
55 |
same as Reverse (17mer) |
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LUCIFERASE PRIMERS |
|
Primer Sequence |
Cat.# |
Tm (°C) |
Applicable Vectors |
|
GLprimer1 (clockwise)
5´-d(TGTATCTTATGGTACTGTAACTG)-3´ |
E1651 |
50 |
pGL2-Basic, -Promoter, -Enhancer, -Control |
|
GLprimer2
(counterclockwise)
5´-d(CTTTATGTTTTTGGCGTCTTCCA)-3´ |
E1661 |
55 |
pSP-luc +; pGL3-Basic, -Promoter,
-Enhancer, -Control; pGL2-Basic,
-Promoter,
-Enhancer, -Control |
|
RVprimer3
(clockwise)
5´-d(CTAGCAAAATAGGCTGTCCC)-3´
|
E4481 |
50 |
pGL3; pCAT®3; pGL4; pCBR-Basic, -Control; pCBG68-Basic, -Control;
pCBG99-Basic,
-Control |
|
RVprimer4
(counterclockwise)
5´-d(GACGATAGTCATGCCCCGCG)-3´ |
E4491 |
60 |
pGL3; pCAT®3; pGL4; pCBR-Basic, -Control; pCBG68-Basic, -Control;
pCBG99-Basic, -Control |
|
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LAMBDA PRIMERS |
|
Primer Sequence |
Cat.# |
Tm (°C) |
Applicable Vectors |
|
Lambda
gt11 Forward
5'-d(GGTGGCGACGACTCCTGGAGCCCG)-3' N/A 69 |
NA |
69 |
Lambda gt11 |
|
Lambda gt11 Reverse
5'd(TTGACACCAGACCAACTGGTAATG)-3' |
NA |
57 |
Lambda gt11 |
|
Lambda gt10
Forward
5'-d(CTTTTGAGCAAGTTCAGCCTGGTTAAG)-3' |
NA |
59 |
Lambda gt10 |
|
Lambda gt10 Reverse
5'-d(GAGGTGGCTTATGAGTATTTCTTCCAGGGTA)-3' |
NA |
55 |
Lambda gt10 |
|
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MISCELLANEOUS SEQUENCING PRIMERS |
|
Primer Sequence |
Cat.# |
Tm (°C) |
Applicable Vectors |
|
PinPoint™ Sequencing Primer
5'-d(CGTGACGCGGTGCAGGGCG)-3' |
V4211 |
66 |
PinPoint™ Xa-1, -2, -3; PinPoint™ Xa-1
T-Vector |
|
Lambda gt11 Reverse
5´-d(CTTTATGTTTTTGGCGTCTTCCA)-3´ |
Q4461 |
55 |
pTARGET™ |
|
|
Melting temperatures (Tm) for each oligonucleotide determined by the
base-stacking free-energy method as listed on Promega's Technical Resources
web site: promega.com/biomath/ NA = Not
available.
Some vectors listed on this page are no longer sold by Promega. The
information is provided here for reference purposes only. |
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