where N is the number of nucleotides and 660pg/pmol is the average MW
of a nucleotide pair.
FORMULAS for DNA MOLAR CONVERSIONS—for ssDNA
To convert pmol to μg:
pmol x N x 330pg x 1μg
= μg
pmol 106pg
To convert μg to pmol:
μg x 106pg x pmol x 1 = pmol
1μg
330pg N
where N is the number of nucleotides and 330pg/pmol is the average MW
of a nucleotide.
PROTEIN MOLAR CONVERSIONS
100pmol of 100KDa
protein
= 10μg
100pmol of 50kDa protein
= 5μg
100pmol of 10kDa
protein
= 1μg
100pmol of 1kDa protein
= 100ng
PROTEIN/DNA CONVERSIONS
1kb of DNA
=
333 amino acids of coding capacity
=
37kDa protein
270bp DNA
=
10kDa protein
810bp DNA
=
30kDa protein
1.35kb DNA
=
50kDa protein
2.7kb DNA
=
100kDa protein
average MW of an amino acid
=
110 daltons
Dalton (Da) is an alternate name for the atomic mass unit, and
kilodalton (kDa) is 1,000 daltons. Thus a peptide with a mass of 64kDa
has a molecular weight of 64,000 grams per mole.
