| Product
(Compound) |
Absorbance (nm) |
Excitation/
Emission
(nm) |
| Apo-ONE® System
(rhodamine 110) |
— |
485/530 |
| AttoPhos®
System (2'-[2-benzothiazoyl]-6'-hydroxybenzothiazole; BBT) |
— |
435/555 |
| β-Galactosidase Enzyme Assay
System (o-nitrophenol) |
420 |
— |
| CaspACE™ Assay
System, Colorimetric (p-nitroaniline; pNA) |
405 |
— |
| CaspACE™ Assay System,
Fluorometric (7-amino-4-methyl coumarin; AMC) |
— |
360/460 |
| CellTiter 96®
Assay (MTT formazan product) |
570 |
— |
| CellTiter 96® AQueous
Assay (MTS formazan product) |
490 |
— |
| CellTiter 96®
AQueous One Solution Assay (MTS formazan product) |
490 |
— |
| CellTiter-Blue®
Assay (resorufin) |
570(1) |
560/590 |
| ChipShot™
Labeling and Clean-Up System: |
|
|
|
|
550 |
550/570 |
|
|
650 |
650/670 |
| CytoTox 96® Assay
(formazan product) |
490 |
— |
| CytoTox-Fluor™
Assay |
— |
485/520 |
| CytoTox-ONE™ Assay (resorufin) |
570 (1) |
560/590 |
| DAPI (nucleic
acid stain) |
— |
360/460 |
| DeadEnd™ Fluorometric TUNEL System
(fluorescein) |
— |
494/520 |
| Emax®
ImmunoAssay Systems (3,3', 5,5'-tetramethylbenzidine; TMB) |
450 |
— |
| FluoroTect™ GreenLys
tRNA (BODIPY®-FL) |
— |
502/510 |
| Greiss Reagent
(azo compound) |
520-550 |
— |
| HaloTag® diAcFAM Ligand
(after hydrolysis) |
— |
494/562 |
| HaloTag®
TMR Ligand |
— |
555/585 |
| HaloTag® Coumarin
Ligand |
— |
353/442 |
| Hemoglobin
(present in rabbit reticulocyte lysates) |
300–600 |
— |
| Monster Green®
Fluorescent Protein (hMGFP) |
— |
480/540 |
|
MultiTox-Fluor Assay |
— |
400/505
and 485/520 |
| Nucleic acids |
260 |
— |
| PepTag™ peptides |
570 |
540/592 |
| Phosphatase assay (molybdate dye) |
600 or 630 |
— |
Polysaccharides
(potential contaminant in DNA preparations) |
230 |
— |
| PowerPlex® Systems : |
|
|
- 6-carboxy-4',5'-dichloro-2',7-dimethoxy-fluorescein
(JOE)
|
— |
520/548 |
- carboxy-tetramethylrhodamine (TMR)
|
— |
550/573 |
- carboxy-X-rhodamine (CXR)
|
— |
578/604
|
|
|
— |
494/520 |
| ProFluor®
Assays : |
|
|
|
|
—
|
485/530 |
- 7-amino-4-methyl-coumarin (AMC)
|
— |
335/460
|
| Propidium iodide (nucleic acid
stain) |
— |
540/620 |
| Protein |
280 |
— |
| (1)Resorufin levels
can be quantitated spectrophotometrically at 570nm, but
measuring fluorescence is recommended due to the increased
sensitivity. See the CellTiter-Blue® Cell
Viability Assay Technical Bulletin, #TB317 or the
CytoTox-ONE™ Homogeneous Membrane Integrity Assay
Technical Bulletin #TB306 for more information. |
