Luciferases are enzymes that generate light through the release of chemical energy in their substrates as photons. This process is luminescence and differs from fluorescence in that a chemical reaction occurs and there is no light needed to excite the molecules involved (excitation).

All luciferases have emission spectra with broad peaks, and thus it is generally recommended that the luminescence be measured over as much of the visible spectrum as possible. Our Chroma-Luc™ technology is an exception to that general rule in that it uses two different luciferases with overlapping but distinct emission spectra. The light emission from each of these luciferases is measured over a different range of wavelengths.

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