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All genes in the bacterium are presumed to be in the wildtype state,
except for those listed, which are mutant alleles carried by that
bacterium. Genes listed on the F' episome, however, represent wildtype
alleles unless specified otherwise. Strains are λ– unless
specified otherwise. |
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BACTERIAL STRAINS, GENOTYPES |
| STRAIN |
GENOTYPE |
| BL21(DE3) |
F–, ompT, hsdSB(rB-, mB-), dcm,
gal, λ(DE3) |
| *BL21(DE3) pLysS |
F–, ompT, hsdSB(rB-, mB-), dcm,
gal, λ(DE3), pLysS (Cmr) |
| *BMH 71-18 mutS |
thi, supE, Δ(lac-proAB), (mutS::Tn10(tetr)], [F', tra D36, proAB,
lacIqZΔM15] |
| C600 (1) |
thi-1, thr-1, leuB6, lacY1, tonA21,
supE44 |
| C600hfl (1) |
thi-1, thr-1, leuB6, lacY1, tonA21,
supE44, hflA150::Tn10(tetr) |
| DH1 (2) |
recA1, endA1, gyrA96, thi-1, hsdR17(rK-, mK+),
supE44, relA1 |
| DH10B |
F–, mcrA Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15, ΔlacX74,
deoR, recA1, endA1,
araD139, Δ(ara, leu)7697, galU, galK, λ–,
rpsL(strr), nupG |
| DH5α™ |
φ80dlacZΔM15, recA1, endA1, gyrAB, thi-1, hsdR17(rK-, mK+),
supE44, relA1, deoR,
Δ(lacZYA-argF) U169,
phoA |
| DM1 (3) |
F', dam-13::Tn9(Cmr) dcm, mcrB, hsdr-M+, gal1,
gal2, ara-, lac-, thr-, leu-, tonR,
tsxR, Suo |
| ES1301 mutS |
lacZ53, thyA36, rha-5, metB1, deoC, IN(rrnD-rrnE), [mutS201::Tn5] |
| *HB101 (4) |
thi-1, hsdS20(rB-, mB-), supE44,
recA13, ara-14, leuB6, proA2, lacY1,
galK2, rpsL20(strr), xyl-5, mtl-1 |
| JM101 (5) |
supE, thi, Δ(lac-proAB), F' (traD36,
proAB, lacIqZΔM15) |
| *JM109 (5) |
endA1, recA1, gyrA96, thi-1, hsdR17(rK-, mK+),
relA1, supE44, Δ(lac-proAB), [F', traD36,
proAB, lacIqZΔM15] |
| JM109(DE3) (5) |
endA1, recA1, gyrA96, thi-1, hsdR17(rK-, mK+),
relA1, supE44, Δ(lac-proAB), [F', traD36,
proAB, lacIqZΔM15], λ(DE3) |
| JM110 (5) |
rpsL(strr), thr, leu, thi, hsdR17(rK-, mK+),
lacY, galK, galT, ara, tonA, tsx,
dam, dcm, supE44, Δ(lac-proAB), [F', traD36,
proAB, lacIqZΔM15] |
| KW251 |
supE44, galK2, galT22, metB1, hsdR2, mcrB1, mcrA, [argA81::Tn10(tetr)],
recD1014 |
| LE392 (6) |
hsdR514, (rK-, mK+),
supE44, supF58, lacY1 or Δ(lacIZY)6, galK2,
galT22, metB1, trpR55 |
| NM522 (7) |
supE, thi, Δ(lac-proAB), Δhsd5(rK-, mK+),
[F', proAB, lacIqZΔM15] |
| NM538 (8) |
supF, hsdR(rK-, mK+),
trpR, lacY |
|
NM539 (8) |
supF, hsdR(rK-, mK+), lacY, (P2) |
| *Select96™ |
mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15,
ΔlacX74, recA1, araD139 (ara-leu)7697,
galU, galK, rspL, endA1, nupG |
| Stbl2™ |
F–, mcrA, Δ(mcrBC-hsdRMS-mrr),
recA1, endA1, gyrA96, thi-1, supE44, relA1,
λ-,
Δ(lac-proAB) |
| Stbl4™ |
mcrA, Δ(mcrBC-hsdRMS-mrr), recA1, endA1,
gyrA96, thi-1, supE44, relA1, λ-,
Δ(lac-proAB), gal, F'{proAB, lacIq, ZΔM15, Tn10(tetR)} |
| SURE® |
e14-, (mcrA-), Δ(mcrCB-hsdSMR-mrr)171,
endA1, supE44, thi-1, gyrA96, relA1,
lac, recB, recJ, sbcC, umuC::Tn5(kanr),
uvrC, [F', proAB, lacIqZΔM15::Tn10(tetr)] |
| TOP10 |
F–, mcrA, Δ(mrr-hsdRMS-mcrBC), φ80lacZΔM15,
ΔlacX74, deoR, recA1, araD139,
Δ(ara, leu)7697, galU, galK, rpsL(strr),
endA1, nupG |
| TOP10F' |
F'{lacIqTn10(tetR)}, mcrA, Δ(mrr-hsdRMS-mcrBC),
φ80lacZΔM15, ΔlacX74, deoR, recA1, araD139,
Δ(ara-leu)7697, galU, galK, rpsL(strr),
endA1, nupG |
| XL1-Blue |
recA1, endA1, gyrA96, thi-1, hsdR17(rK-, mK+),
supE44, relA1, lac,
[ F', proAB, lacIqZΔM15::Tn10(tetr)] |
| XL10-Gold® |
Tetr, Δ(mcrA)183, Δ(mcrCB-hsdSMR-mrr)173, endA1,
supE44, thi-1, recA1, gyrA96, relA1,
lac Hte, [ F', proAB, lacIqZΔM15 ,Tn10(tetr)
Amy Camr]a |
|
Y1089 (9) |
Δ(lacU169), proA+, Δ(lon), araD139, strA,
hflA150, [chr::Tn10(tetr)], (pMC9) |
| Y1090 (9) |
Δ(lacU169), proA+, Δ(lon), araD139, strA,
supF, rpsL(strr), [trpC22::Tn10(tetr)], (pMC9),
hsdR(rK-, mK+) |
| *Strains available from Promega as competent cells are
indicated by an asterisk. Strains shown in bold are available from
Promega as glycerol freezer stocks. |
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MISCELLANEOUS and REFERENCES |
- F' host contains an F' episome with the stated features.
- λ(DE3) is bacteriophage λ carrying the gene for T7 RNA polymerase
integrated into the host genome.
- pMC9 is pBR322 with lacIq inserted and confers ampicillin and
tetracycline resistance.
References
- Jendrisak, J., Young, R.A. and Engel, J. (1987) In: Guide to
Molecular Cloning Techniques, Berger, S. and Kimmel, A. eds.,
Academic Press, San Diego, CA.
- Hanahan, D. (1983) J. Mol. Biol. 166, 557.
- Lorow-Murray, D. and Bloom, F. (1991) Focus 13,
20.
- Lacks, S. and Greenberg, J.R. (1977) J. Mol. Biol. 114,
153.
- Yanisch-Perron, C., Vieira, J. and Messing, J. (1985) Gene
33, 103.
- Murray, N. Brammar, W.J. and Murray, K. (1977) Mol. Gen.
Genet. 150, 53.
- Gough, J. and Murray, N. (1983) J. Mol. Biol. 166,
1.
- Frischauf, A. et al. (1983) J. Mol. Biol. 170,
827.
- Huynh, T., Young, R.A. and Davis, R. (1985) In: DNA Cloning
, Vol. 1, Glover, D., ed., IRL Press Ltd., Oxford, UK.
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