Transcription Factors:
Recombinant Human AP1
(c-Jun) (Cat.# E3061)
is the protein encoded by the human proto-oncogene, c-jun. Its expression is greatly modulated by extracellular stimuli such as phorbol ester and growth factor treatment. It is a member of the AP1 family.
c-Jun is expressed in E. coli from a human cDNA clone and has a molecular weight of 40kDa.
Recombinant Human NF-κB (p50) (Cat.# E3770)
is expressed in bacteria from a human cDNA derived from the full-length p105. p50, a processed product of its p105 precursor, is the first DNA-binding subunit of the NF-κB transcription factor to be described. The amino-terminal half of p50 has strong homology to the proto-oncogene, c-rel, and its viral counterpart, v-rel, and the Drosophila dorsal gene as well.
Recombinant Human SP1 (rhSP1) (Cat.# E6391)
is expressed from a human baculovirus cDNA clone in Sf9 cells. The SP1 transcriptional control sequence is found in a large number of viral and cellular promoters, including the HIV long terminal repeat. The SP1 transcription factor contains contiguous zinc finger motifs that provide specific DNA binding activity. SP1 factor interacts with other coactivator proteins that can modulate transcriptional activity.
Recombinant Human TFIIB (rhTFIIB) (Cat.# E3790)
is a general transcription factor involved in formation of an in vitro active complex capable of specifically initiating RNA synthesis by RNA polymerase II.
Transcription Factor Consensus Oligonucleotides:
SP1, AP1,
AP2, TFIID,
NF-κB, OCT1
and CREB
Technical Articles:
Gel shift analysis with Human
Recombinant AP1 (c-jun):
The effect of poly d(I-C) on specific complex formation (PN037)
Transcriptional activation assays
of recombinant transcription factors (PN034)
Using SAM2® Biotin Capture Membranes to Study Protein:DNA
Binding (eNotes, AP0043)
|
