In Vitro Transcription, Transcription Regulation & Microarrays Technical Resources
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RNA Analysis Guide
The "RNA Analysis Notebook" provides an introduction to procedures such as RNA purification, amplifying via RT-PCR, making RNA in vitro and analyzing RNA by microarray. In addition, the "RNA Analysis Notebook" highlights Promega products for use in performing these procedures. This notebook is available from the Guides web, as a pdf. You can download the entire guide, or select the chapter that best suits your research interests.
Microarrays Section, Protocols & Applications Guide
The section on microarrays, "Techniques to Monitor Gene Expression Levels", in the Expression Analysis chapter (Chapter 6) of the Protocols & Applications Guide, describes the limitations of Northern blots and RNase protection assays and the benefits of microarrays, with a brief analysis of how they are performed. See the link below, then scroll to the Microarrays section.
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