Imaging & Immunological Detection Technical Resources

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The HaloTag® Interchangeable Labeling Technology allows you to assess expression of your protein in a live of fixed cells, or in prepared lysates.

The HaloTag® Technology can simplify multicolor experiments for live-cell imaging. Since the HaloTag® protein is not an inherently fluorescent protein (IFP), the choice of fluorescent labels, including secondary and tertiary fluorophores, can be made after creating the HaloTag® fusion protein. This feature allows more flexibility in the choice of fluorophores.

HaloTag® Ligands List

Label proteins in fixed cells and combine with ICC for two-color imaging

HaloTag® ligands retain their fluorescent properties after fixation, allowing the possibility of performing multiplexing experiments that employ immunocytochemistry (ICC).

List of available antibodies

Emax® ImmunoAssay Systems

There are six different Emax ImmunoAssay Systems available to detect the presence of brain-derived neurotrophic factor (BDNF), glial cell-line-derived neurotrophic factor (GDNF), nerve growth factor (NGF), neurotrophin-3 (NT-3) and transforming growth factors β1 (TGFβ1) and β2 (TGFβ2) in many species including human, mouse and rat. These systems are:

BDNF Emax® ImmunoAssay System GDNF Emax® ImmunoAssay System NGF Emax® ImmunoAssay System NT-3 Emax® ImmunoAssay System TGFβ1 Emax® ImmunoAssay System TGFβ2 Emax® ImmunoAssay System

Anti-ACTIVE® Antibodies

The Anti-ACTIVE® Antibodies are specific for the active forms of caspase-3, JNK, CaM KII, MAPK and p38 with the accessory Anti-ACTIVE®-Qualified Anti-Rabbit conjugates. These antibodies can be used for Western blotting (except for Anti-ACTIVE® Caspase-3), immunocytochemistry and immunohistochemistry applications when specifically detecting the active form of the enzyme is important.

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Protocols and Applications Guide

The Cell Imaging chapter of the Protocols and Applications Guide discusses various systems for live-cell and in vivo imaging. Here you will find product details, example protocols, discussions of specific  technologies, and more..

Protocols and Applications Guide...