Imaging & Immunological Detection Technical Resources
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The HaloTag® Interchangeable Labeling Technology allows you to assess expression
of your protein in a live of fixed cells, or in prepared lysates.
The HaloTag® Technology can simplify multicolor experiments for live-cell imaging. Since the HaloTag® protein is not an inherently fluorescent protein (IFP), the choice of fluorescent labels, including secondary and tertiary fluorophores, can be made after creating the HaloTag® fusion protein. This feature allows more flexibility in the choice of fluorophores.
HaloTag® Ligands ListLabel proteins in fixed cells and combine with ICC for two-color imaging
HaloTag® ligands retain their fluorescent properties after fixation, allowing the possibility of performing multiplexing experiments that employ immunocytochemistry (ICC).
List of available antibodiesEmax® ImmunoAssay Systems
There are six different Emax ImmunoAssay Systems available to detect the presence of brain-derived neurotrophic factor (BDNF), glial cell-line-derived neurotrophic factor (GDNF), nerve growth factor (NGF), neurotrophin-3 (NT-3) and transforming growth factors β1 (TGFβ1) and β2 (TGFβ2) in many species including human, mouse and rat. These systems are:
BDNF Emax® ImmunoAssay System GDNF Emax® ImmunoAssay System NGF Emax® ImmunoAssay System NT-3 Emax® ImmunoAssay System TGFβ1 Emax® ImmunoAssay System TGFβ2 Emax® ImmunoAssay SystemAnti-ACTIVE® Antibodies
The Anti-ACTIVE® Antibodies are specific for the active forms of caspase-3, JNK, CaM KII, MAPK and p38 with the accessory Anti-ACTIVE®-Qualified Anti-Rabbit conjugates. These antibodies can be used for Western blotting (except for Anti-ACTIVE® Caspase-3), immunocytochemistry and immunohistochemistry applications when specifically detecting the active form of the enzyme is important.
Learn more...Protocols and Applications Guide
The
Cell Imaging chapter of the Protocols and
Applications Guide discusses various systems for
live-cell and in vivo imaging. Here you will find product details, example protocols, discussions of specific technologies, and more..