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DNA & RNA Purification

Sample Types Processed with the Wizard® SV Genomic DNA Purification System
DNA from the following sample types has been successfully purified at Promega or by external laboratories using the Wizard® SV Genomic DNA Purification System (Cat.# A2360, A2361).
 

Many of these protocols have been communicated to Promega by external laboratories. Because these protocols have not been performed at Promega, they are intended to be guidelines that might require optimization.

This list will be updated as additional information becomes available. Contact Promega Technical Services (techserv@promega.com) for the latest information on available protocols.

Sample Types Processed Using the Wizard® SV Genomic DNA Purification System.
Sample Type Promega External Comments
Blood and blood cells
  Whole blood Yes Yes A protocol is provided in the Application Notes #AN101.
Reference:
Researchers isolated bacterial DNA from whole blood samples inoculated with Neisseria meningitidis, Streptococcus pneumoniae and Haemophilus influenzae.
Smith, K., Diggle, M.A. and Clarke, S.C. (2003) J. Clin. Microbiol. 41, 2440–3. PubMed ID #12791861
  Peripheral blood mononuclear cells Yes Ezzeldin, H.H. et al. (2005) Clin. Cancer Res. 11,
8699–705. PubMed ID #16361556.
  Mouse primary granulocytes, macrophages, B lymphocytes and T lymphocytes Yes Morii, E. et al. (2004) Blood 104, 1656–61.
PubMed ID #15172970
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Sample Type Promega External Comments
Mammalian tissues
  Mouse tail clipping Yes Yes A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
Reference:
Smith, P. et al. (2005) J. Exp. Med. 202, 1319–25.
PubMed ID #16301741.
  Mouse liver Yes A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
  Mouse brain Yes   A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
  Mouse heart Yes   A protocol is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302.
  Mouse spleen Yes Fakhari, F.D. et al. (2006) J. Clin. Invest. 116, 735–42. PubMed ID #16498502.
  Mouse ear Yes Du, Q. et al. (2006) FASEB J. 20, 1131–41.
PubMed ID #16770012.
  Mouse skin   Yes Basal cell carcinoma and noncancerous tissues were frozen at –80 °C prior to DNA isolation.
Mancuso, M. et al. (2006) Cancer Res. 66, 6606–14. PubMed ID #16818633.
  Anopheles funestus mosquito carcass Yes Michel, A.P. et al. (2006) Genetics 173, 1389–95.
PubMed ID #16648581.
  Human lung cancer biopsy Yes Sasaki, H. et al. (2005) Clin. Cancer Res. 11, 2924–9.
PubMed ID #15837743.
  Human distant normal colorectal mucosa and tumor tissue Yes Pfeifer, D. et al. (2005) Carcinogenesis 26, 103–7.
PubMed ID #15485994.
  Human frozen thymic epithelial tumor samples   Yes Suzuki, E. et al. (2006) Jpn. J. Clin. Oncol. 36, 351–6. PubMed ID #16762968.
  Human pancreatic islet   Yes Del Guerra, S. et al. (2005) Diabetes 54, 727–35.
PubMed ID #15734849.
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Sample Type Promega External Comments
Cultured cells
(Established cell lines are not listed.)		 Yes   A protocol for DNA isolation from mammalian cultured cells is provided in the Wizard® SV Genomic DNA Purification System Technical Bulletin #TB302. Any additional resources are listed below.
  Chicken retinospheroid cultures Yes Paraoanu, L.E. et al. (2006) Toxicol. Sci. 89, 314–24. PubMed ID #16207942.
  HT1080 human fibrosarcoma Yes Cells were transfected with FIV vectors, and genomic DNA was isolated 3 days post-transduction.
Harper, S.Q. et al. (2006) J. Virol. 80, 9371–80.
PubMed ID #16973543.
  PLC/PRF/5 human hepatoma Yes Cells were infected with ~1 multiplicity of infection (MOI) of pseudotyped HCV and total DNA isolated.
Blanchard, E. et al. (2006) J. Virol. 80, 6964–72.
PubMed ID #16809302.
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Sample Type Promega External Comments
Bacteria
  Bacillus macyae Yes Cells in mid- to late exponential phase were centrifuged for 20 minutes at 21,000 × g at 4 °C.
Santini, J.M. et al. (2004) Int. J. Syst. Evol. Microbiol. 54, 2241–4. PubMed ID #15545465.
  E. coli K-12 derivative, MC4100 Yes Teresa Pellicer, M. et al. (2003) J. Bacteriol. 185, 5815–21. PubMed ID #13129953.
  Pseudomonas aeruginosa Yes Zhu, H. et al. (2004) Invest. Ophthalmol. Vis. Sci. 45, 1897–903. PubMed ID #15161855.
  Bacillus subtilis Yes Park, J.H. et al. (2004) J. Bacteriol. 186, 1571–3.
PubMed ID #14973012.
  Staphylococcus sp.   Yes A modified Wizard® SV Genomic Purification System protocol was used.
McDonald, R. and Hill, O. (2004) Isolation of genomic DNA from bacterial cells using the Wizard® SV Genomic DNA Purification System. eNotes
  Enterococcus faecalis   Yes A modified Wizard® SV Genomic Purification System protocol was used.
McDonald, R. and Hill, O. (2004) Isolation of genomic DNA from bacterial cells using the Wizard® SV Genomic DNA Purification System. eNotes
  Listeria monocytogenes   Yes A modified Wizard® SV Genomic Purification System protocol was used.
McDonald, R. and Hill, O. (2004) Isolation of genomic DNA from bacterial cells using the Wizard® SV Genomic DNA Purification System. eNotes
  Salmonella sp.   Yes A modified Wizard® SV Genomic Purification System protocol was used.
McDonald, R. and Hill, O. (2004) Isolation of genomic DNA from bacterial cells using the Wizard® SV Genomic DNA Purification System. eNotes
  Escherichia coli O157:H7   Yes A modified Wizard® SV Genomic Purification System protocol was used. Follow link below for specific modifications.
McDonald, R. and Hill, O. (2004) Isolation of genomic DNA from bacterial cells using the Wizard® SV Genomic DNA Purification System. eNotes
  Eleven Gram-negative bacterial species   Yes Modified the eNotes protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400 µl of enzymatic lysis solution (47 mM EDTA, 25 mg/ml lysozyme, 20 µg/ml lysostaphin) and incubated for 2 hours at 37 °C. Next, 19.2 mg/ml proteinase K was added (final concentration 0.4 mg/ml), and the mixture was incubated for 1 hour at 55 °C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80 °C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9. PubMed ID #16891488.
  Eleven Gram-positive bacterial species   Yes Modified the eNotes protocol using the Wizard® SV Genomic Purification System to isolate bacteria as follows: The pelleted bacteria were resuspended in 400 µl of enzymatic lysis solution (47 mM EDTA, 25 mg/ml lysozyme, 20 µg/ml lysostaphin) and incubated for 2 hours at 37 °C. Next, 19.2 mg/ml proteinase K was added (final concentration 0.4 mg/ml), and the mixture was incubated for 1 hour at 55 °C. Finally, the Nuclei Lysis Solution and RNase Solution were added, mixed and incubated for 10 minutes at 80 °C.
Zucol, F. et al. (2006) J. Clin. Microbiol. 44, 2750–9. PubMed ID #16891488.
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Sample Type Promega External Comments
Other organisms and tissues
  Schistosoma mansoni (blood fluke) worm   Yes Smith, P. et al. (2004) J. Immunol. 173, 1240–8.
PubMed ID #15240716.
  Snail cercariae   Yes Smith, P. et al. (2004) J. Immunol. 173, 1240–8.
PubMed ID #15240716.
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