This list will be updated as additional information becomes available. Contact
Promega Technical Services (techserv@promega.com)
for the latest information on available protocols.
|
Sample Types Processed Using
the PureYield™ RNA Midiprep System. |
| Sample Type |
Promega |
External |
Comments |
|
Blood and blood cells |
| |
Human blood |
Yes |
|
A protocol is provided in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
|
Mammalian tissues |
|
|
A general protocol for total RNA isolation from tissue is
provided in the PureYield™ RNA Midiprep System
Technical Manual #TM279.
Additional resources are listed below. |
| |
Rat liver |
Yes |
|
Follow the standard protocol for tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
| |
Rat lung |
Yes |
|
Follow the
standard protocol for tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
| |
Rat kidney |
Yes |
|
Follow the standard protocol for tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
| |
Rat spleen |
Yes |
|
Follow the
standard protocol for tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
| |
Rat brain |
Yes |
|
Follow the standard protocol for tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
| |
Rat heart |
Yes |
|
Follow the
standard protocol for tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
| |
Rat muscle |
Yes |
|
Follow the standard protocol for tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
| |
Bovine liver |
Yes |
|
Follow the
standard protocol for tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| |
Bovine spleen |
Yes |
|
Follow the standard protocol for tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| |
Bovine heart |
Yes |
|
Follow the
standard protocol for tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| Top |
|
Plant tissues |
|
|
A general protocol for total RNA isolation from plant tissue is
provided in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. Additional
resources are listed below. |
| |
Canola leaf |
Yes |
|
Follow the standard
protocol for plant tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
| |
Corn/maize leaf,
stem
and silk tissue |
Yes |
Yes |
Follow the
standard protocol for plant tissue in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| Top |
Cultured cells
|
Yes |
|
A general protocol for total RNA isolation from
mammalian cultured cells is provided in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| Top |
|
Bacteria |
|
|
A general protocol for total RNA isolation from
Gram-positive and Gram-negative bacteria is provided in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| |
Escherichia coli |
Yes |
|
Follow the standard protocol for bacteria in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
| |
Bacillus subtilis |
Yes |
|
Follow the
standard protocol for bacteria in the
PureYield™ RNA Midiprep System Technical Manual
#TM279. |
| |
Bacillus sphaericus |
Yes |
|
Follow the standard protocol for bacteria in the
PureYield™ RNA Midiprep System Technical Manual
#TM279. |
| |
Bacillus globigii |
Yes |
|
Follow the
standard protocol for bacteria in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| |
Artherobacter luteus |
Yes |
|
Follow the standard protocol for bacteria in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| |
Micrococcus luteus |
Yes |
|
Follow the
standard protocol for bacteria in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| |
Staphylococcus aureus |
Yes |
|
Follow the standard protocol for bacteria in the
PureYield™ RNA Midiprep System Technical Manual
#TM279. |
| Top |
|
Other organisms and tissues |
| |
Saccharomyces cerevisiae |
Yes |
|
A protocol is provided in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Brisco, P. et al. (2006)
Promega Notes 92, 14–9. |
| |
Chicken liver |
Yes |
|
Follow the
standard tissue protocol found in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| Top |
|
Miscellaneous |
| |
phenol:chloroform-extracted RNA samples |
Yes |
|
A protocol for clean up of phenol:chloroform-extracted RNA samples is provided in the
PureYield™ RNA Midiprep System Technical
Manual #TM279.
Additional resource
Mandrekar, M. et al. (2006) Promega Notes 94, 7–11. |
| |
RNA pellet from an
alcohol precipitation |
Yes |
|
A protocol for
clean up of RNA pellets is provided in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| |
Purified RNA in an aqueous solution |
Yes |
|
A protocol for cleaning up RNA in an
aqueous solution is provided in the
PureYield™ RNA Midiprep System Technical
Manual #TM279. |
| Top |
|
