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DNA & RNA Purification

Sample Types Processed Using the PolyATtract® System 1000

RNA from the following sample types has been successfully purified at Promega or by external laboratories using the PolyATtract® System 1000.
 

Many of these protocols have been communicated to Promega by external laboratories. Because these protocols have not been performed at Promega, they are intended to be recommendations that might require optimization.

This list will be updated as additional information becomes available. Contact Promega Technical Services (techserv@promega.com) for the latest information on available protocols.

Sample Types Processed Using the PolyATtract® System 1000.
Sample Type Promega External Comments
Blood and blood cells Yes   A protocol is provided in the article Kephart, D. (1997) Promega Notes 62, 11–13. Additional resources are listed below.
  Human blood mononuclear cells Yes Singleton, B.K. et al. (2000) Blood 95, 12–8.
PubMed ID #10607679
  Human peripheral blood lymphocytes Yes Meyer-Koenig, U. et al. (2006) J. Clin. Microbiol. 44, 3394–6. PubMed ID #16954283
  Rhesus macaque peripheral blood mononuclear cells Yes Schaefer, T.M. et al. (2000) J. Virol. 74, 3273–83. PubMed ID #10708444
  Winter flounder red blood cells Yes Pedersen, S.F. et al. (2003) Am. J. Physiol. Cell. Physiol. 284, C1561–76. PubMed ID #12734109
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Mammalian tissues Yes   A general protocol for mRNA isolation from tissue is provided in the PolyATtract® System 1000 Technical Manual #TM228. Additional resources are listed below.
  Baboon placental villous tissue and syncytiotrophoblast-enriched cell fractions Yes Henson, M.C. et al. (1997) Endocrinology 138, 1385–91. PubMed ID #9075692.
  Canine cardiac tissue Yes Lee, Y.A. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 11035–40. PubMed ID #8855304
  Human bladder tumor tissue Yes Wild, P.J. et al. (2005) Clin. Cancer Res. 11, 4415–29. PubMed ID #15958626
  Human high-grade prostatic intraepithelial neoplasia Yes Hu, Y. et al. (2007) Clin. Cancer Res. 13, 929–38. PubMed ID #17289887.
  Human laser-microdissected breast and breast cancer tissue Yes Dahl, E. et al. (2006) Clin. Cancer Res. 12, 3950–60. PubMed ID #16818692
  Human liver Yes Pollak, E.S. et al. (1996) J. Biol. Chem. 271, 1738–47. PubMed ID #8576177
  Mouse intestinal mucosa Yes Kutuzova, G.D. et al. (2006) Proc. Natl. Acad. Sci. USA 103, 12377–81. PubMed ID #16895982
  Mouse lacrimal gland Yes For glandular tissues with high lipid content, the mRNA isolation protocol was modified as follows: centrifuge the tissue homogenates, then add N-lauroylsarcosine (to a final concentration of 0.3%) to the supernatant. Incubate the mixture at 70°C with biotinylated oligo-d(T).
Reference:
Remington, S.G., Lima, P.H. and Nelson, J.D. (1999) Invest. Ophthalmol. Vis. Sci. 40, 1081–90.
PubMed ID #10235541
  Mouse spleen Yes Albert, T.J. et al. (2003) Nucleic Acids Res. 31, e35. PubMed ID #12655023
  Ovine lung tissue Yes Pietschmann, S.M. and Pison, U. (2000) Am. J. Physiol. Lung Cell. Mol. Physiol. 278, L765–78.
PubMed ID #10749754
  Rat hypothalamus Yes Hu, Y. et al. (1996) J. Biol. Chem. 271, 26315–9. PubMed ID #8824284
  Rat liver Yes Gardmo, C., Persson, B. and Mode, A. (2001) Endocrinology 142, 2695–701. PubMed ID #11356721
  Rat mammary carcinoma tissue Yes Ariazi, E.A. and Gould, M.N. (1996) J. Biol. Chem. 271, 29286–94. PubMed ID #8910589
  Rat myometrium Yes Engstrøm, T. et al. Biol. Reprod. 60, 322–9.
PubMed ID #9915997
  Rat psoas muscle, lung, heart, liver, brain, kidney and reticulocyte Yes Mellor, H. et al. (1994) J. Biol. Chem. 269, 10201–4. PubMed ID #7908290
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Plant tissues   Yes A protocol is provided in the article Murillo, I. et al. (1995) Promega Notes 54, 2–7.
  Arabidopsis seedlings Yes Yuen, C.Y. et al. (2003) Plant Physiol. 131, 493–506. PubMed ID #12586874.
  Maize endosperm Yes Jia, H. et al. (2007) Crop Sci. 47, S-45–S-59.
  Marchantia polymorpha Yes Nagai, J. et al. (1999) DNA Res. 6, 1–11.
PubMed ID #10231024.
  Medicago truncatula embryo tissue Yes Bouton, S. et al. (2005) J. Exp. Bot. 56, 825–32.
PubMed ID #15689340.
  Pisum sativum L. Yes Martin, D.N., Proebsting, W.M. and Hedden, P. (1997) Proc. Natl. Acad. Sci. USA 94, 8907–11.
PubMed ID #9238076.
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Cultured cells
 		 Yes Yes A general protocol for mRNA isolation from cells is provided in the PolyATtract® System 1000 Technical Manual #TM228.
References:
Wild, P.J. et al. (2005) Mol. Cancer Ther. 4, 516–28. PubMed ID #15827324.
Wallqvist, A. et al. (2006) Mol. Pharmacol. 69, 737–48.
PubMed ID #16332985.
  H9 cells chronically infected with HIV Yes Bourara, K., Litvak, S. and Araya, A. (2000) Science 289, 1564–6. PubMed ID #10968794.
  Human primary dermal fibroblast Yes Hu, B. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 10008–13. PubMed ID #12913118.
  Human primary dermal microvascular endothelial cells Yes Hartmeyer, M. et al. (1997) J. Immunol. 159, 1930–7.
PubMed ID #9257858.
  Human umbilical vein endothelial cells Yes Kendall, R.L. and Thomas, K.A. (1993) Proc. Natl. Acad. Sci. USA 90, 10705–9. PubMed ID #8248162.
  Human primary skin fibroblasts Yes Jong, M.T. et al. (1999) Hum. Mol. Genet. 8, 783–93.
PubMed ID #10196367.
  IMR90 (human fibroblast cell line) Yes Ferbeyre, G. et al. (2000) Genes Dev. 14, 2015–27.
PubMed ID #10950866.
  NIH 3T3 (mouse embryonic fibroblast) Yes Larsson, O. et al. (2006) Nucleic Acids Res. 34,
4375–86. PubMed ID #16936314.
  RK13 (rabbit kidney cells) infected with South American myxoma virus Yes Jackson, R.J., Hall, D.F. and Kerr, P.J. (1999) J. Virol. 73, 2376–84. PubMed ID #9971821.
  Rabbit primary parietal cells Yes Parente, J.A. Jr. et al. (1999) J. Biol. Chem. 274,
3017–25. PubMed ID #9915840.
  Six human and monkey cell lines (TE-671, JEG-3, OVCAR-3, SK-OV-3, MCF-7, COS-7) Yes Cheng, C.K. et al. (2003) Mol. Endocrinol. 17, 1175–91.
PubMed ID #12663744.
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Other organisms and tissues
  Cryphonectria parasitica Yes Zhang, L., Baasiri, R.A. and Van Alfen, N.K. (1998) Mol. Cell. Biol. 18, 953–9. PubMed ID #9447992.
  Dictyostelium cells Yes Iranfar, N. et al. (2001) Mol. Cell. Biol. 12, 2590–600. PubMed ID #11553701.
  Drosophila heads Yes Gengs, C. et al. (2002) J. Biol. Chem. 277, 42113–20. PubMed ID #12196539.
  Entamoeba histolytica Yes Gilchrist, C.A. et al. (2001) J. Biol. Chem. 276,
11838–43. PubMed ID #11278344.
  Phormia regina (blowfly) antennae Yes Tsuchihara, K. et al. (2005) Chem. Senses 30, 559–64. PubMed ID #16107518.
  Schistosoma japonicum (blood fluke) Yes Blood fluke worms were frozen and ground with a mortar and pestle in liquid nitrogen. The ground tissue was transferred to a sterile 50ml conical tube and the liquid nitrogen allowed to evaporate. Guanidine thiocyanate extraction buffer was added to the sample and mRNA isolated directly from the suspension.
Reference:
Waine, G.J. et al. (1993) Infect. Immun. 61, 4716–23. PubMed ID #8406870.
  Schizosaccharomyces pombe (fission yeast) Yes Akamatsu, Y. et al. (2003) Proc. Natl. Acad. Sci. USA 100, 15770–5. PubMed ID #14663140.
  Trimeresurus jerdonii (pit viper) venom glands Yes Sanz, L. et al. (2005) J. Biol. Chem. 280, 40714–22. PubMed ID #16215260.
  White spot bacilliform virus (isolated from infected shrimp) Yes Yang, F. et al. (2001) J. Virol. 75, 11811–20.
PubMed ID #11689662.
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