Cloning Technical Resources
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Promega cloning products include restriction enzymes, modifying enzymes (ligases, PNK, alkaline phosphatases), subcloning vectors, DNA & RNA polymerases and DNA markers.
Use the menu on the right to find articles, product guides, protocols, vector sequences and other cloning information.
Single Step Competent Cells
Single Step (KRX) Competent Cells can be used for cloning and screening, and allow tightly controlled protein expression. Find out more in the following articles:
The Single Step (KRX) Competent Cells: Efficient Cloning and High Protein Yields Protein Expression in Less Time: A Short Induction Protocol for KRX Compatibility of Single Step (KRX) Competent Cells with the MagneGST™ Pull-Down SystemNew Cloning Resource
The cloning chapter of the Protocols and Applications Guide covers cloning using PCR and restriction enzymes, including DNA clean-up, ligation, transformation and screening to identify recombinant clones.
Restriction Enzymes Resource Guide
The Restriction Enzymes Resource Guide contains useful interactive searches and reference information including:
- Information on specific enzymes, enzymes generating compatible ends, enzymes by recognition sequence, and suitable buffers for double-digests.
- Restriction Enzyme Reaction Buffer Composition
- Restriction Enzyme Activity in Promega 10X Buffers
- Heat inactivation and methylation sensitivity of Promega restriction enzymes