Abstract for PowerPlex^® Matrix Standards, 3100—Custom

Part# TMD029
Printed in USA 8/09.
Instructions for Use of Product X3121.

Proper generation of a spectral calibration file is critical to evaluate multicolor systems with the ABI PRISM^® 3100 and 3100-Avant and Applied Biosystems 3130 and 3130xl Genetic Analyzers. The PowerPlex^® Matrix Standards, 3100—Custom, includes individual fragments labeled with four different fluorescent dyes. Each matrix fragment is in a separate tube: one tube contains a 375bp fragment labeled with fluorescein (FL), two tubes each contain a 350bp fragment labeled with 6-carboxy-4´,5´-dichloro-2´,7´-dimethoxyfluorescein (JOE), one tube contains a 300bp fragment labeled with carboxytetramethylrhodamine (TMR), and one tube contains a 275bp fragment labeled with carboxy-X-rhodamine (CXR). These matrix standards include both JOE A- and JOE B-labeled fragments, but this protocol uses only JOE A.