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Abstract for PowerPlex® 16 HS System
The PowerPlex® 16 HS System allows co-amplification and three-color detection
of sixteen loci (fifteen STR loci and Amelogenin), including Penta E, D18S51, D21S11, TH01, D3S1358,
FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818. One primer
for each of the Penta E, D18S51, D21S11, TH01 and D3S1358 loci is labeled with fluorescein (FL);
one primer for each of the FGA, TPOX, D8S1179, vWA and Amelogenin loci is labeled with
carboxy-tetramethylrhodamine (TMR); and one primer for each of the Penta D, CSF1PO, D16S539, D7S820,
D13S317 and D5S818 loci is labeled with 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxy-fluorescein
(JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed in a single injection.
The PowerPlex® 16 HS System provides all of the materials necessary for amplification
of these loci, including hot-start Taq DNA polymerase, and is compatible with
the ABI PRISM® 310, 3100 and 3100-Avant and Applied Biosystems 3130
and 3130xl Genetic Analyzers.
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