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Abstract for PowerPlex® 16 BIO System
The PowerPlex® 16 BIO System allows the coamplification and
three-color detection of sixteen loci (fifteen STR loci and Amelogenin). The
PowerPlex® 16 BIO System contains the loci Penta E, D18S51, D21S11,
TH01, D3S1358, FGA, TPOX, D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539,
D7S820, D13S317 and D5S818. In this system, one primer specific for Penta E,
D18S51, D21S11, TH01 and D3S1358 is labeled with fluorescein (FL); one primer
specific for FGA, TPOX, D8S1179, vWA and Amelogenin is labeled with Rhodamine
Red™-X; and one primer specific for Penta D, CSF1PO, D16S539, D7S820, D13S317
and D5S818 is labeled with 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxy-fluorescein
(JOE). All sixteen loci are amplified simultaneously in a single tube and
analyzed in a single gel lane.
The PowerPlex® 16 BIO System is designed specifically for use
with the Hitachi FMBIO® II Fluorescence Imaging System. It provides
all of the materials necessary for amplification of STR regions of purified
genomic DNA except for AmpliTaq Gold® DNA polymerase.
This manual contains separate protocols for use of the PowerPlex® 16
BIO System with the Perkin-Elmer model 480 and GeneAmp® PCR system
9600, 9700 and 2400 thermal cyclers in addition to protocols for separation and
detection of amplified materials. |
