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Abstract for PowerPlex® 16 System
The PowerPlex® 16 System allows the co-amplification and
three-color detection of sixteen loci (fifteen STR loci and Amelogenin). The
system contains the loci Penta E, D18S51, D21S11, TH01, D3S1358, FGA, TPOX,
D8S1179, vWA, Amelogenin, Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818.
One primer specific for Penta E, D18S51, D21S11, TH01 and D3S1358 is labeled
with fluorescein (FL); one primer specific for FGA, TPOX, D8S1179, vWA and
Amelogenin is labeled with carboxy-tetramethylrhodamine (TMR); and one primer
specific for Penta D, CSF1PO, D16S539, D7S820, D13S317 and D5S818 is labeled
with 6-carboxy-4′,5′-dichloro-2′,7′-dimethoxy-fluorescein
(JOE). All sixteen loci are amplified simultaneously in a single tube and analyzed
in a single injection or gel lane.
The PowerPlex® 16 System is designed specifically for use with
the ABI PRISM® 310 Genetic Analyzer and is compatible with the ABI
PRISM® 3100 and 3100-Avant Genetic Analyzers and 377 DNA
Sequencer and Applied Biosystems 3130 and 3130xl Genetic Analyzers.
The PowerPlex® 16 System provides all of the materials necessary
for amplification of STR regions of purified genomic DNA with the exception of
AmpliTaq Gold® DNA polymerase. This manual contains separate
protocols for use of the PowerPlex® 16 System with the Perkin-Elmer
model 480 and GeneAmp® PCR system 9600, 9700 and 2400 thermal
cyclers in addition to protocols for separation of amplified products and detection
of separated material.
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