Abstract for PowerPlex® 1.2 System
Part# TMD009
Printed in USA. Revised 6/09.
Instructions for Use of Product DC6101.
The PowerPlex® 1.2 System allows co-amplification
and two-color detection of nine loci (eight STR loci and
Amelogenin). The system includes the STR loci found in
the GenePrint® GammaSTR® System (D16S539, D7S820, D13S317
and D5S818) and GenePrint® Fluorescent STR Multiplex—CSF1PO, TPOX,
TH01 and vWA (CTTv Multiplex), as well as the GenePrint® Fluorescent Sex
Identification System—Amelogenin (TMR). All nine loci are amplified simultaneously in a
single tube and analyzed in a single capillary injection or gel lane.
The PowerPlex® 1.2 System is designed specifically for use with the
ABI PRISM® 310 Genetic Analyzer and is compatible with the ABI PRISM®
377 DNA Sequencer and ABI PRISM® 3100 Genetic Analyzer. The PowerPlex®
1.2 System provides all materials necessary for amplification of STR regions of purified genomic
DNA except AmpliTaq Gold® DNA polymerase. This manual contains separate protocols
for the Perkin-Elmer model 480 and GeneAmp® PCR system 2400, 9600 and 9700
thermal cyclers in addition to protocols to separate amplified products and detect separated material.
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