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Abstract for PowerPlex® 1.2 System
The PowerPlex®
1.2 System allows the coamplification and two-color detection of nine loci (eight STR loci and Amelogenin). The system
contains all the component STR loci of two GenePrint® quadriplex systems, the
GenePrint® GammaSTR® System (D16S539, D7S820,
D13S317 and D5S818) and the GenePrint® Fluorescent STR
Multiplex-CSF1PO, TPOX, TH01 and vWA (CTTv Multiplex), as well as the GenePrint® Fluorescent Sex
Identification System-Amelogenin (TMR). One of the two primers for each locus in
the GammaSTR® Multiplex is labeled with fluorescein (FL); one primer is specific for each locus in the
CTTv Multiplex and one primer for Amelogenin is labeled with
carboxy-tetramethylrhodamine (TMR). All nine loci
are amplified simultaneously in a single tube and analyzed in a single capillary injection or gel lane.
The PowerPlex®
1.2 System is designed specifically for use with the ABI PRISM®
310 Genetic Analyzer and is compatible with the ABI PRISM®
377 DNA Sequencer and ABI PRISM® 3100 Genetic Analyzer. The PowerPlex® 1.2 System
provides all of the materials necessary for amplification of STR regions of purified genomic DNA
with the exception of AmpliTaq Gold® DNA polymerase. This manual
contains separate protocols for use of the PowerPlex® 1.2 System with the
Perkin-Elmer model 480 and GeneAmp® PCR system 2400, 9600 and
9700 thermal cyclers in addition to protocols for separation of amplified
products and detection of separated material.
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