Abstract for PowerPlex^® 1.2 System

Part# TMD009
Printed in USA. Revised 6/09.
Instructions for Use of Product DC6101.

The PowerPlex^® 1.2 System allows co-amplification and two-color detection of nine loci (eight STR loci and Amelogenin). The system includes the STR loci found in the GenePrint^® GammaSTR^® System (D16S539, D7S820, D13S317 and D5S818) and GenePrint^® Fluorescent STR Multiplex—CSF1PO, TPOX, TH01 and vWA (CTTv Multiplex), as well as the GenePrint^® Fluorescent Sex Identification System—Amelogenin (TMR). All nine loci are amplified simultaneously in a single tube and analyzed in a single capillary injection or gel lane.

The PowerPlex^® 1.2 System is designed specifically for use with the ABI PRISM^® 310 Genetic Analyzer and is compatible with the ABI PRISM^® 377 DNA Sequencer and ABI PRISM^® 3100 Genetic Analyzer. The PowerPlex^® 1.2 System provides all materials necessary for amplification of STR regions of purified genomic DNA except AmpliTaq Gold^® DNA polymerase. This manual contains separate protocols for the Perkin-Elmer model 480 and GeneAmp^® PCR system 2400, 9600 and 9700 thermal cyclers in addition to protocols to separate amplified products and detect separated material.