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Abstract for PowerPlex® 1.2 System

The PowerPlex® 1.2 System allows the coamplification and two-color detection of nine loci (eight STR loci and Amelogenin). The system contains all the component STR loci of two GenePrint® quadriplex systems, the GenePrint® GammaSTR® System (D16S539, D7S820, D13S317 and D5S818) and the GenePrint® Fluorescent STR Multiplex-CSF1PO, TPOX, TH01 and vWA (CTTv Multiplex), as well as the GenePrint® Fluorescent Sex Identification System-Amelogenin (TMR). One of the two primers for each locus in the GammaSTR® Multiplex is labeled with fluorescein (FL); one primer is specific for each locus in the CTTv Multiplex and one primer for Amelogenin is labeled with carboxy-tetramethylrhodamine (TMR). All nine loci are amplified simultaneously in a single tube and analyzed in a single capillary injection or gel lane.

The PowerPlex® 1.2 System is designed specifically for use with the ABI PRISM® 310 Genetic Analyzer and is compatible with the ABI PRISM® 377 DNA Sequencer and ABI PRISM® 3100 Genetic Analyzer. The PowerPlex® 1.2 System provides all of the materials necessary for amplification of STR regions of purified genomic DNA with the exception of AmpliTaq Gold® DNA polymerase. This manual contains separate protocols for use of the PowerPlex® 1.2 System with the Perkin-Elmer model 480 and GeneAmp® PCR system 2400, 9600 and 9700 thermal cyclers in addition to protocols for separation of amplified products and detection of separated material.


Protocols
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Part# TMD009
Printed in USA. Revised 6/07.
Instructions for Use of Product DC6101: Request this protocol.

Printed publications disclaimer.